Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.25 extracted from

  • Fong, K.P.Y.; Goh, C.B.H.; Tan, H.M.
    Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase (1996), J. Bacteriol., 178, 5592-5601.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas putida enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth catechol + CO2 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ML2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
89.1
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth Pseudomonas putida catechol + CO2 + NADH
-
 ?
1,2-propanediol + NAD+ at 189% of the activity with cis-benzene dihydrodiol Pseudomonas putida ?
-
 ?
1,2-propanediol + NAD+ at 189% of the activity with cis-benzene dihydrodiol Pseudomonas putida ML2 ?
-
 ?
cis-1,2-cyclohexanediol + NAD+ at 95% of the activity with cis-benzene dihydrodiol Pseudomonas putida ?
-
 ?
cis-benzene dihydrodiol + NAD+
-
 Pseudomonas putida dihydroxybenzene + NADH
-
 ?
glycerol + NAD+ at 18% of the activity with cis-benzene dihydrodiol Pseudomonas putida ?
-
 ?
glycerol + NAD+ at 18% of the activity with cis-benzene dihydrodiol Pseudomonas putida ML2 ?
-
 ?