Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.24 extracted from

  • Noguchi, M.; Yoshida, T.; Kikuchi, G.
    Purification and properties of biliverdin reductases from pig spleen and rat liver (1979), J. Biochem., 86, 833-848.
    View publication on PubMed
Search for more literature for 1.3.1.24

Activating Compound

Activating Compound Comment Organism Structure
albumin
-
 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Arsenobenzoate 1 mM, slight Sus scrofa
Biliverdin
-
 Rattus norvegicus
Biliverdin substrate inhibition Sus scrofa
FAD
-
 Sus scrofa
FMN
-
 Sus scrofa
iodoacetamide
-
 Rattus norvegicus
iodoacetamide 1 mM, slight Sus scrofa
N-ethylmaleimide
-
 Rattus norvegicus
N-ethylmaleimide 1.0 mM Sus scrofa
p-chloromercuribenzoate
-
 Rattus norvegicus
p-chloromercuribenzoate 0.025 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001 0.002 Biliverdin cosubstrate NADH Sus scrofa
0.003
-
 Biliverdin cosubstrate NADPH, rat liver Sus scrofa
0.003 0.005 NADPH pig spleen Sus scrofa
1.5 2 NADH pig spleen, rat liver Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 gel filtration Sus scrofa
34000
-
 1 * 34000, multiple forms, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biliverdin + NAD(P)H Sus scrofa importance in linear tetrapyrrole metabolism bilirubin + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Rattus norvegicus
-
spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity Rattus norvegicus
-20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin + NAD(P)H
-
 Rattus norvegicus bilirubin + NAD(P)+
-
 ?
biliverdin + NAD(P)H pig spleen: IXalpha-biliverdin: most effective substrate among the 4 biliverdin isomers Sus scrofa bilirubin + NAD(P)+
-
 ?
biliverdin + NAD(P)H not: IXalpha-biliverdin methylester (pig spleen) Sus scrofa bilirubin + NAD(P)+
-
 ?
biliverdin + NAD(P)H pig spleen: strictly specific to biliverdin, no other oxidoreductase activities Sus scrofa bilirubin + NAD(P)+
-
 ?
biliverdin + NAD(P)H importance in linear tetrapyrrole metabolism Sus scrofa bilirubin + NAD(P)+
-
 ?
biliverdin + NADH + H+
-
 Sus scrofa bilirubin + NAD+
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 34000, multiple forms, SDS-PAGE Rattus norvegicus
monomer 1 * 34000, multiple forms, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 biliverdin + NADH Sus scrofa
8.5
-
 biliverdin + NADPH Rattus norvegicus
8.5
-
 biliverdin + NADPH Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Rattus norvegicus
NADH
-
 Sus scrofa
NADPH
-
 Rattus norvegicus
NADPH
-
 Sus scrofa