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Literature summary for 1.3.1.22 extracted from
- Reduced nicotinamide adenine dinucleotide phosphate delta4-3-ketosteroid 5alpha-oxidoreductase (rat ventral prostate) (1975), Methods Enzymol., 36A, 466-474.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM: less than 20% inhibition | Rattus norvegicus |  |
| Cd2+ | 1 mM: less than 20% inhibition | Rattus norvegicus | |
| Cu2+ | 1 mM | Rattus norvegicus | |
| Mn2+ | 1 mM: less than 20% inhibition | Rattus norvegicus | |
| Zn2+ | 1 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
testosterone | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
| nuclear envelope | - |
Rattus norvegicus | 5635 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epididymis | - |
Rattus norvegicus | - |
| genitalia | external | Rattus norvegicus | - |
| additional information | highest activity in organs of acessory reproduction and in liver | Rattus norvegicus | - |
| preputial gland | - |
Rattus norvegicus | - |
| prostate gland | ventral | Rattus norvegicus | - |
| seminal vesicle | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months | Rattus norvegicus |
| 4°C, 2 days, 50% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxyprogesterone + NADPH | - |
Rattus norvegicus | 17alpha-hydroxy-5alpha-pregnan-3,20-dione + NADP+ | - |
ir | |
| 20-alpha-hydroxypregn-4-ene-3one + NADPH | - |
Rattus norvegicus | 20-alpha-hydroxy-5alpha-pregnan-3-one + NADP+ | - |
ir | |
| androstenediol + NADPH | - |
Rattus norvegicus | 5alpha-androstan-3beta,17beta-diol + NADP+ | - |
ir | |
| androstenedione + NADPH + H+ | - |
Rattus norvegicus | 5alpha-androstan-3,17-dione + NADP+ | - |
ir | |
| cholest-4-en-3-one + NADPH | mammalian enzymes: no reverse reaction, first step in reverse direction (enolization) occurs, but energy barrier for hydride abstraction is extremely high | Rattus norvegicus | 5-alpha-cholestan-3-one + NADP+ | - |
ir | |
| cortexolone + NADPH | - |
Rattus norvegicus | 17alpha,21-dihydroxy-5alpha-pregnan-3,20-dione + NADP+ | - |
ir | |
| deoxycorticosterone + NADPH | - |
Rattus norvegicus | 21-hydroxy-5alpha-pregnan-3,20-dione | - |
ir | |
| epitestosterone + NADPH | - |
Rattus norvegicus | ? | - |
ir | |
| progesterone + NADPH + H+ | - |
Rattus norvegicus | 5alpha-pregnan-3,20-dione + NADP+ | - |
ir | |
| testosterone + NADPH + H+ | - |
Rattus norvegicus | 17beta-hydroxy-5alpha-androstan-3-one + NADP+ | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
1 h stable | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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