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Literature summary for 1.3.1.21 extracted from
- Cholesterol biosynthesis from lanosterol: development of a novel assay method, characterization, and solubilization of rat hepatic microsomal sterol DELTA7-reductase (1997), J. Biochem. Mol. Biol., 30, 370-377.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-[p-(beta-Diethylaminoethoxy) phenyl]1-(p-tolyl)-2-(p-chlorophenyl)ethanol | i.e. MER-29 or triparanol | Rattus norvegicus |  |
| 3beta-[2-(diethylamino)ethoxy]androst-5-en-17-one | i.e. U18666A | Rattus norvegicus | |
| ergosterol | non-competetively, concentration 0.025 mM | Rattus norvegicus | |
| trans-1,4-Bis-(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride | i.e. AY-9944, hypercholesterolaemic drug | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
ergosterol | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesta-5,7-dien-3-beta-ol + NADPH | Rattus norvegicus | - |
cholesterol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00011 | - |
testis enzyme, extract | Rattus norvegicus |
| 0.00108 | - |
solubilized by 1.5% 3-[3-(cholamidopropyl)-dimethylammonio]-1-propanesulfonate, i.e. CHAPS, from microsomes | Rattus norvegicus |
| 0.0023 | - |
liver enzyme, extract | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesta-5,7-dien-3-beta-ol + NADPH | - |
Rattus norvegicus | cholesterol + NADP+ | - |
? | |
| cholesta-5,7-dien-3beta-ol + NADPH | - |
Rattus norvegicus | cholesterol + NADP+ | - |
? | |
| cholesta-7-en-3beta-ol + NADPH | low activity | Rattus norvegicus | ? | - |
? | |
| cholesta-7-en-3beta-ol + NADPH | i.e. lathosterol | Rattus norvegicus | ? | - |
? | |
| ergosta-5,7,22-trien-3beta-ol + NADPH | i.e. ergosterol | Rattus norvegicus | ergosta-5,22-diene-3beta-ol + NADP+ | - |
? | |
| ergosta-5,7,22-trien-3beta-ol + NADPH | enzyme activity diet-inducible: 2.6fold by 0.5% ergosterol and 5fold by 5.0% cholestyramine and 0.1% lovastatin | Rattus norvegicus | ergosta-5,22-diene-3beta-ol + NADP+ | - |
? | |
| ergosterol + NADP+ | - |
Rattus norvegicus | ? | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sterol DELTA7-reductase | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000109 | - |
trans-1,4-Bis-(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride | - |
Rattus norvegicus | |
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