Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
complexed with NADP+ and testosterone, 10fold molar excess of NADP+, 'waterbug' dialysis method in 10 mM potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM 2-mrcapthoethanol, 5% acetonitrile and 0.2 mM testosterone, and crystallization in presence of polethylene glycol 8000 in hanging drops at room temperature, single crystals | Rattus norvegicus |
cryocrystallization: crystals were soaked in 21% polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.4, 1.25 mM NADP+, 0.1 mM testosterone and 15% 2-methyl-2,4-pentanediol as cryoprotectant | Rattus norvegicus |
structure determination, cofactor and substrate binding site, and model building | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37029 | - |
1 * 37029, sequence determination | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADPH + H+ | Rattus norvegicus | - |
5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | structure analysis reveals that the enzyme belongs to the aldo-keto reductase superfamily, like 3alpha-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, aldose reductase, and aldehyde reductase | Rattus norvegicus | |
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | enzyme termed 3alpha-hydroxysteroid/dihydrodiol dehydrogenase, structure-based catalytic mechanism analysis, ordered bi-bi mechanism | Rattus norvegicus | |
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | cofactor binding and catalytic triad, alpha/beta-barrel structure | Rattus norvegicus | |
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | termed AKR1C as member of the aldo-keto-superfamily, overview | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-keto steroid + NAD(P)H | - |
Rattus norvegicus | 3alpha-hydroxy steroid + NAD(P)+ | - |
r | |
5alpha-dihydrotestosterone + NADPH + H+ | - |
Rattus norvegicus | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37029, sequence determination | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |