Application | Comment | Organism |
---|---|---|
medicine | enzyme can be used for screening of antiinflammatory potency of drugs | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
vapour diffusion at 22°C in 100 mM sodium citrate, polyethylene glycol 6000 25%, 120 mM ammonium sulfate, pH 5.8, x-ray analysis | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C170A | kinetically similar to wild-type | Rattus norvegicus |
C217A | 4fold increase in Km for 5alpha-androstan-3,17-dione and 2fold increase in Km for NADH | Rattus norvegicus |
additional information | sequence analysis | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition by nonsteroidal antiinflammatory drugs | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37029 | - |
1 * 37029, cDNA sequence determination | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | reduces biologically important 3-oxo steroids of the androstane, pregnane, and cholane series | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | cofactor binding and catalytic triad, alpha/beta-barrel structure | Rattus norvegicus | |
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | enzyme is multifunctional: 3alpha-hydroxysteroid dehydrogenase activity, EC 1.1.1.50, dihydrodiol dehydrogenase activity, EC 1.3.1.20, and 9-, 11-, and15-hydroxyprostaglandin dehydrogenase activity, belongs to aldo-keto reductase superfamily | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-keto steroid + NAD(P)H | reduces biologically important 3-oxo steroids of the androstane, pregnane, and cholane series | Rattus norvegicus | 3alpha-hydroxy steroid + NAD(P)+ | - |
? | |
additional information | reduces biologically important 3-oxo steroids of the androstane, pregnane, and cholane series | Rattus norvegicus | ? | - |
? | |
additional information | oxidation of trans-dihydrodiols of polycyclic hydrocarbons and in this way suppression of formation of anti-diol epoxid carcinogens | Rattus norvegicus | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37029, cDNA sequence determination | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus |