BRENDA - Enzyme Database show
show all sequences of 1.3.1.20

Roles of His-79 and Tyr-180 of D-xylose/dihydrodiol dehydrogenase in catalytic function

Asada, Y.; Aoki, S.; Ishikura, S.; Usami, N.; Hara, A.; Biochem. Biophys. Res. Commun. 278, 333-337 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type and mutants, H79Q and Y180F, in Escherichia coli
Macaca fuscata
Engineering
Amino acid exchange
Commentary
Organism
H79Q
site-directed mutagenesis, moderate activity with 190fold increase in Km-value, inhibition by MgCl2 and potassium phosphate
Macaca fuscata
additional information
sequence analysis reveals high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes
Macaca fuscata
Y180F
site-directed mutagenesis, very low activity
Macaca fuscata
Inhibitors
Inhibitors
Commentary
Organism
Structure
MgCl2
inhibition of mutant enzymes H79Q and Y180F
Macaca fuscata
phosphate buffer
inhibitionn of mutant enzymes H79Q and Y180F
Macaca fuscata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
Camphorquinone
NADPH
Macaca fuscata
0.51
-
Camphorquinone
mutant H79Q, NADPH
Macaca fuscata
0.55
-
NADP+
phosphate buffer 0.1 M
Macaca fuscata
0.56
-
NADPH
-
Macaca fuscata
2.1
-
NADP+
Tris buffer 25 mM
Macaca fuscata
4.4
-
D-xylose
NADP+, 25 mM Tris buffer 25 mM
Macaca fuscata
6.3
-
D-xylose
NADP+, 0.1 M phosphate buffer
Macaca fuscata
14
-
NADP+
mutant H79Q, phosphate buffer 0.1 M
Macaca fuscata
28
-
NADPH
mutant H79Q
Macaca fuscata
67
-
D-xylose
mutant H79Q, NADP+, 0.1 M phosphate buffer
Macaca fuscata
213
-
D-xylose
mutant H79Q, NADP+, Tris buffer 25 mM
Macaca fuscata
402
-
NADP+
mutant H70Q, Tris buffer 25 mM
Macaca fuscata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, SDS-PAGE
Macaca fuscata
70000
-
gel filtration
Macaca fuscata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Macaca fuscata
-
japanese monkey
-
Purification (Commentary)
Commentary
Organism
wild-type and mutant protein recombinant from Escherichia coli
Macaca fuscata
Reaction
Reaction
Commentary
Organism
trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+
identical with D-xylose dehydrogenase EC 1.1.1.179
Macaca fuscata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Macaca fuscata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
camphorquinone + NAD(P)H
-
286127
Macaca fuscata
(1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
-
-
?
D-xylose + NADP+
-
286127
Macaca fuscata
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
additional information
monomeric forms are identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.50 and provide a broad substrate specificity, while dimeric forms are identical with D-xylose dehydrogenase EC 1.1.1.179 and are active with trans-dihydrodiols and dicarbonyl compounds
286127
Macaca fuscata
additional information
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 36000, SDS-PAGE
Macaca fuscata
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Macaca fuscata
NADPH
-
Macaca fuscata
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutants, H79Q and Y180F, in Escherichia coli
Macaca fuscata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Macaca fuscata
NADPH
-
Macaca fuscata
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H79Q
site-directed mutagenesis, moderate activity with 190fold increase in Km-value, inhibition by MgCl2 and potassium phosphate
Macaca fuscata
additional information
sequence analysis reveals high sequence homology between dimeric mammalian dihydrodiol dehydrogenases, but no homology with monomeric or cis-dihydrodiol-specific enzymes
Macaca fuscata
Y180F
site-directed mutagenesis, very low activity
Macaca fuscata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
MgCl2
inhibition of mutant enzymes H79Q and Y180F
Macaca fuscata
phosphate buffer
inhibitionn of mutant enzymes H79Q and Y180F
Macaca fuscata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
Camphorquinone
NADPH
Macaca fuscata
0.51
-
Camphorquinone
mutant H79Q, NADPH
Macaca fuscata
0.55
-
NADP+
phosphate buffer 0.1 M
Macaca fuscata
0.56
-
NADPH
-
Macaca fuscata
2.1
-
NADP+
Tris buffer 25 mM
Macaca fuscata
4.4
-
D-xylose
NADP+, 25 mM Tris buffer 25 mM
Macaca fuscata
6.3
-
D-xylose
NADP+, 0.1 M phosphate buffer
Macaca fuscata
14
-
NADP+
mutant H79Q, phosphate buffer 0.1 M
Macaca fuscata
28
-
NADPH
mutant H79Q
Macaca fuscata
67
-
D-xylose
mutant H79Q, NADP+, 0.1 M phosphate buffer
Macaca fuscata
213
-
D-xylose
mutant H79Q, NADP+, Tris buffer 25 mM
Macaca fuscata
402
-
NADP+
mutant H70Q, Tris buffer 25 mM
Macaca fuscata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, SDS-PAGE
Macaca fuscata
70000
-
gel filtration
Macaca fuscata
Purification (Commentary) (protein specific)
Commentary
Organism
wild-type and mutant protein recombinant from Escherichia coli
Macaca fuscata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Macaca fuscata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
camphorquinone + NAD(P)H
-
286127
Macaca fuscata
(1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
-
-
?
D-xylose + NADP+
-
286127
Macaca fuscata
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
additional information
monomeric forms are identical with 3alpha-hydroxysteroid dehydrogenase EC 1.1.1.50 and provide a broad substrate specificity, while dimeric forms are identical with D-xylose dehydrogenase EC 1.1.1.179 and are active with trans-dihydrodiols and dicarbonyl compounds
286127
Macaca fuscata
additional information
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 36000, SDS-PAGE
Macaca fuscata
Other publictions for EC 1.3.1.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740358
Liu
Metabolites analysis, metaboli ...
Ruditapes philippinarum
Ecotoxicol. Environ. Saf.
107
251-259
2014
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1
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2
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1
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1
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1
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2
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1
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697571
Chang
Overexpression of dihydrodiol ...
Homo sapiens
Digest. Dis. Sci.
54
342-347
2009
-
1
-
-
-
-
-
-
1
-
-
1
-
1
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2
1
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1
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1
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1
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1
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2
1
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1
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684150
Carbone
Structure of monkey dimeric di ...
Macaca fascicularis, Macaca fuscata
Acta Crystallogr. Sect. D
64
532-542
2008
-
-
2
2
30
-
4
26
-
-
-
2
-
2
-
-
2
-
-
-
-
-
4
2
-
-
-
10
-
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-
3
-
-
23
-
-
2
3
2
30
-
23
4
-
26
-
-
-
2
-
-
-
2
-
-
-
-
4
2
-
-
-
10
-
-
-
-
-
-
-
-
-
-
686023
Carbone
Structural and functional feat ...
Bos taurus, Macaca fascicularis, Mus musculus, Pongo abelii, Sus scrofa
Cell. Mol. Life Sci.
65
1464-1474
2008
-
-
-
1
5
-
8
-
-
2
-
-
-
5
-
-
-
-
-
3
-
-
15
2
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-
10
-
-
2
-
-
-
10
1
5
-
2
8
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2
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3
-
-
15
2
-
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701119
Carbone
Structures of dimeric dihydrod ...
Macaca fascicularis
Proteins Struct. Funct. Genet.
70
176-187
2008
-
-
1
1
3
-
1
-
-
-
1
1
-
1
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1
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3
-
1
2
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-
1
-
1
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-
2
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1
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1
2
1
3
-
1
1
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1
1
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-
1
-
-
3
-
1
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
685913
Pan
Dihydrodiol dehydrogenase in d ...
Homo sapiens
Cancer Chemother. Pharmacol.
59
697-702
2007
-
-
-
-
-
-
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1
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1
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1
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1
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2
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1
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-
-
-
687141
Wang
Reversal of inflammation-assoc ...
Homo sapiens
Int. J. Cancer
120
2019-2027
2007
1
-
-
-
-
-
3
-
-
-
1
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-
2
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-
1
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2
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1
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2
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2
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6
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2
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-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689972
Carbone
-
Structures of dimeric dihydrod ...
Macaca fascicularis
Proteins Struct. Funct. Bioinform.
70
176-187
2007
-
-
1
1
3
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
1
-
-
-
-
1
-
-
1
-
-
1
1
1
3
-
1
1
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-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
671358
Hung
Expression of dihydrodiol dehy ...
Homo sapiens
Anticancer Res.
26
2949-2956
2006
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
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-
1
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-
1
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-
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-
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6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673371
Cheng
A possible role for dihydrodio ...
Homo sapiens
Environ. Mol. Mutagen.
48
14-21
2006
-
1
-
-
1
-
-
-
-
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1
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3
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1
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1
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3
-
-
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-
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-
-
-
-
675089
Chow
Expression of dihydrodiol dehy ...
Homo sapiens
J. Dermatol. Sci.
41
205-212
2006
-
1
-
-
-
-
-
-
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1
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1
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1
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1
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-
-
-
673903
Chen
Overexpression of dihydrodiol ...
Homo sapiens
Gynecol. Oncol.
97
110-117
2005
-
1
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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655159
Deng
Ubiquitous induction of resist ...
Homo sapiens
Cancer Chemother. Pharmacol.
54
301-307
2004
-
-
-
-
-
-
7
-
-
-
-
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-
1
-
-
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-
1
5
-
1
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-
-
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1
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-
1
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-
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7
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1
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655236
Seike
Oxidative DNA damage induced b ...
Mus musculus
Chem. Res. Toxicol.
17
1445-1451
2004
-
1
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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655744
Wang
Inverse expression of dihydrod ...
Homo sapiens
Int. J. Cancer
111
246-251
2004
-
1
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-
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-
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-
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1
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1
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-
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657392
Tang
Hydroxychavicol modulates benz ...
Homo sapiens
Toxicol. Lett.
152
235-243
2004
-
-
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1
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1
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1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
656885
Yang
Reduction of dihydrodiol dehyd ...
Homo sapiens
Oncol. Rep.
10
271-276
2003
-
1
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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-
349258
El-Kabbani
Crystallization and preliminar ...
Macaca fuscata
Acta Crystallogr. Sect. D
58
163-164
2002
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
1
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1
1
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1
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-
1
1
1
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-
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-
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1
-
1
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1
1
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-
-
-
-
655321
Higaki
Molecular characterization of ...
Macaca fascicularis, Macaca fuscata fuscata
Drug Metab. Pharmacokinet.
17
348-356
2002
-
-
-
-
-
-
-
10
-
-
2
-
-
4
-
-
-
-
-
2
2
-
19
2
2
-
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10
2
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1
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1
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2
2
-
19
2
2
-
-
10
2
-
-
-
-
-
-
-
-
-
656043
Deng
Increased expression of dihydr ...
Homo sapiens
J. Biol. Chem.
277
15035-15043
2002
-
-
-
-
1
-
2
-
-
-
-
-
-
1
-
-
-
-
-
2
9
-
3
-
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1
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2
9
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286128
Aoki
Identity of dimeric dihydrodio ...
Canis sp., Homo sapiens, Macaca fuscata, Oryctolagus cuniculus, Sus scrofa
Chem. Biol. Interact.
130-132
775-784
2001
-
-
-
-
-
-
9
10
-
-
-
-
-
5
-
-
3
1
-
11
9
-
29
-
5
-
-
-
-
-
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10
-
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9
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3
-
11
9
-
29
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
349250
Terada
Site-directed mutagenesis stud ...
Bos taurus
Chem. Biol. Interact.
130-132
833-845
2001
-
-
1
-
7
-
3
-
-
-
-
1
-
1
-
-
1
1
-
1
1
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1
-
3
-
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-
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-
4
-
-
-
-
-
-
-
-
-
286127
Asada
Roles of His-79 and Tyr-180 of ...
Macaca fuscata
Biochem. Biophys. Res. Commun.
278
333-337
2000
-
-
1
-
3
-
2
12
-
-
2
-
-
1
-
-
1
1
-
1
-
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3
1
-
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2
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1
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3
1
-
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-
-
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-
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-
-
-
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-
-
349252
Kume
Characterization of a novel va ...
Homo sapiens
Pharmacogenetics
9
763-771
1999
1
-
1
-
3
-
7
-
-
-
1
1
-
1
-
-
1
1
-
1
2
-
13
1
1
-
-
8
-
-
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1
-
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1
-
1
1
-
3
-
-
7
-
-
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1
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-
1
-
1
2
-
13
1
1
-
-
8
-
-
-
-
-
-
-
-
-
-
349260
Burczynski
Expression and characterizatio ...
Homo sapiens
Biochemistry
37
6781-6790
1998
-
-
1
-
-
-
-
12
-
-
-
-
-
1
-
-
1
1
-
1
4
-
6
-
-
-
-
14
-
-
-
1
-
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1
1
-
-
-
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-
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12
-
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1
-
1
4
-
6
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
349261
Matsuura
Roles of the C-terminal domain ...
Homo sapiens
Biochem. J.
336
429-436
1998
4
-
1
-
8
-
7
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
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-
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-
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349262
Matsuura
Identification of amino acid r ...
Homo sapiens
Biochem. J.
323
61-64
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
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-
-
-
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-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349263
Bennett
Steroid recognition and regula ...
Rattus norvegicus
Structure
5
799-812
1997
-
-
1
1
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
1
-
-
2
1
-
-
-
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1
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1
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349259
Hara
Relationship of human liver di ...
Homo sapiens
Biochem. J.
313
373-376
1996
-
-
1
-
-
-
9
-
-
-
-
-
-
1
-
-
1
-
-
1
1
-
5
-
-
-
-
6
-
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2
-
-
-
-
-
1
2
-
-
-
-
9
-
-
-
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-
-
-
-
-
1
-
1
1
-
5
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
349253
Miyabe
Kinetic studies of the inhibit ...
Homo sapiens
Biol. Pharm. Bull.
18
9-12
1995
-
-
-
-
-
-
7
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
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-
2
6
-
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2
-
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-
7
6
-
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1
-
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1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349255
Deyashiki
Expression and kinetic propert ...
Homo sapiens
J. Biochem.
118
285-290
1995
-
-
1
-
-
-
13
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
15
1
-
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-
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2
-
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1
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13
2
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-
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-
-
-
-
1
1
-
15
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349256
Nanjo
Enzymic characterization of a ...
Bos taurus
Biochim. Biophys. Acta
1244
53-61
1995
-
-
-
-
-
-
1
14
1
-
-
-
-
1
-
-
1
-
-
1
-
-
11
-
-
-
-
14
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
14
1
-
-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
349254
Sato
Purification and characterizat ...
Canis sp.
J. Biochem.
116
711-717
1994
-
-
-
-
-
-
8
-
-
-
2
-
-
1
-
-
1
-
-
9
1
-
24
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
8
-
-
-
-
2
-
-
-
-
1
-
9
1
-
24
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349257
Hoog
Three-dimensional structure of ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
91
2517-2521
1994
-
1
-
1
3
-
1
-
-
-
1
1
-
1
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
1
3
-
-
1
-
-
-
-
1
1
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349251
Nanjo
Unique dihydrodiol specific de ...
Bos taurus
Adv. Exp. Med. Biol.
328
371-377
1993
-
-
-
-
-
-
5
4
-
-
-
-
-
1
-
-
1
1
-
1
-
-
6
-
1
-
-
-
-
-
-
1
4
-
-
-
-
-
1
-
-
-
-
5
4
4
-
-
-
-
-
-
-
1
-
1
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
349237
Nakagawa
Dimeric dihydrodiol dehydrogen ...
Macaca fascicularis, Macaca fuscata
J. Biochem.
106
1104-1109
1989
-
-
-
-
-
-
-
9
-
-
2
3
-
2
-
-
1
-
-
2
2
-
14
1
2
-
-
9
2
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
9
-
-
2
3
-
-
-
1
-
2
2
-
14
1
2
-
-
9
2
-
-
-
-
-
-
-
-
-
349238
Penning
Non-K-region o-quinones as enz ...
Rattus norvegicus
Biochemistry
28
4505-4511
1989
-
-
-
-
-
-
2
-
1
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349239
Nakagawa
Identification of two dihydrod ...
Mus musculus
Biochem. J.
106(4)
633-638
1989
-
-
-
-
-
-
9
7
1
-
1
4
-
1
-
-
1
1
-
6
-
-
16
1
1
-
2
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
9
-
7
1
-
1
4
-
-
-
1
-
6
-
-
16
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
349240
Hara
Isolation from pig lens of two ...
Sus scrofa
Biochem. J.
264
403-407
1989
3
-
-
-
-
-
12
-
-
-
2
2
-
1
-
-
1
1
-
4
-
-
7
2
1
-
-
-
4
-
-
4
-
-
-
3
-
-
4
-
-
-
-
12
-
-
-
-
2
2
-
-
-
1
-
4
-
-
7
2
1
-
-
-
4
-
-
-
-
-
-
-
-
-
349241
Smithgall
Spectroscopic identification o ...
Rattus norvegicus
J. Biol. Chem.
263
1814-1820
1988
-
-
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349242
Hara
Purification and partial chara ...
Macaca fuscata
Biochem. Biophys. Res. Commun.
145
1260-1266
1987
-
-
-
-
-
-
-
6
-
-
1
1
-
1
-
-
1
-
-
2
2
-
11
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
6
-
-
1
1
-
-
-
1
-
2
2
-
11
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
349243
Matsuura
Purification and properties of ...
Cavia porcellus
Biochim. Biophys. Acta
912
270-277
1987
3
-
-
-
-
-
8
-
-
-
3
1
-
1
-
-
1
1
-
1
1
-
17
1
-
-
-
-
-
-
-
2
-
-
-
3
-
-
2
-
-
-
-
8
-
-
-
-
3
1
-
-
-
1
-
1
1
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349244
Smithgall
Regio- and stereospecificity o ...
Rattus norvegicus
J. Biol. Chem.
261
6184-6191
1986
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349245
Boutin
Rat liver microsomal 3alpha-hy ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
135
795-801
1986
-
-
-
-
-
-
1
-
2
-
-
1
-
1
-
-
1
1
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349246
Bolcsak
Purification of mouse liver be ...
Mus musculus
J. Biol. Chem.
258
7252-7255
1983
-
-
-
-
-
-
-
1
-
-
6
1
-
1
-
-
1
-
-
-
1
-
5
1
-
-
-
-
3
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
6
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
349247
Vogel
Rat liver cytoplasmic dihydrod ...
Rattus norvegicus
J. Biol. Chem.
255
9621-9625
1980
-
-
-
-
-
-
-
1
1
-
1
1
-
1
-
-
1
-
-
1
1
-
4
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
1
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-