BRENDA - Enzyme Database
show all sequences of 1.3.1.19

Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2

Fong, K.P.Y.; Tan, H.M.; FEBS Lett. 451, 5-9 (1999)

Data extracted from this reference:

General Stability
General Stability
Organism
unstable
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
cis-1,2-dihydrobenzene-1,2-diol
cosubstrate NAD+
Pseudomonas putida
0.22
-
NAD+
-
Pseudomonas putida
46
-
glycerol
cosubstrate NAD+
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions requirement
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
4 * 39000, SDS-PAGE
Pseudomonas putida
166000
-
PAGE
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida
-
catechol + NADH
-
Pseudomonas putida
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida ML2
-
catechol + NADH
-
Pseudomonas putida ML2
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ML2
-
-
-
Purification (Commentary)
Commentary
Organism
dye-affinity chromatography and NAD+ agarose chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
68
-
glycerol, NAD+
Pseudomonas putida
153
-
cis-1,2-dihydrobenzene-1,2-diol, NAD+
Pseudomonas putida
313
-
cis-1,2-cyclohexanediol, NAD+
Pseudomonas putida
513
-
1,2-propanediol, NAD+
Pseudomonas putida
Storage Stability
Storage Stability
Organism
less stable at 4C than at -20C, loss of activity at 4C gradually over a few days
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-propanediol + NAD+
-
390589
Pseudomonas putida
propen-1,2-diol + NADH
-
-
-
?
1,2-propanediol + NAD+
-
390589
Pseudomonas putida ML2
propen-1,2-diol + NADH
-
-
-
?
cis-1,2-cyclohexanediol + NAD+
-
390589
Pseudomonas putida
cyclohex-1-ene-1,2-diol + NADH
-
-
-
?
cis-1,2-cyclohexanediol + NAD+
-
390589
Pseudomonas putida ML2
cyclohex-1-ene-1,2-diol + NADH
-
-
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
390589
Pseudomonas putida
catechol + NADH
-
390589
Pseudomonas putida
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
390589
Pseudomonas putida ML2
catechol + NADH
-
390589
Pseudomonas putida ML2
?
glycerol + NAD+
-
390589
Pseudomonas putida
?
-
-
-
?
glycerol + NAD+
-
390589
Pseudomonas putida ML2
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 39000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
enzyme assay
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
with NAD+ as cofactor, 100 mM potassium carbonate buffer
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
General Stability (protein specific)
General Stability
Organism
unstable
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
cis-1,2-dihydrobenzene-1,2-diol
cosubstrate NAD+
Pseudomonas putida
0.22
-
NAD+
-
Pseudomonas putida
46
-
glycerol
cosubstrate NAD+
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions requirement
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
4 * 39000, SDS-PAGE
Pseudomonas putida
166000
-
PAGE
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida
-
catechol + NADH
-
Pseudomonas putida
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
Pseudomonas putida ML2
-
catechol + NADH
-
Pseudomonas putida ML2
?
Purification (Commentary) (protein specific)
Commentary
Organism
dye-affinity chromatography and NAD+ agarose chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
68
-
glycerol, NAD+
Pseudomonas putida
153
-
cis-1,2-dihydrobenzene-1,2-diol, NAD+
Pseudomonas putida
313
-
cis-1,2-cyclohexanediol, NAD+
Pseudomonas putida
513
-
1,2-propanediol, NAD+
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
less stable at 4C than at -20C, loss of activity at 4C gradually over a few days
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-propanediol + NAD+
-
390589
Pseudomonas putida
propen-1,2-diol + NADH
-
-
-
?
1,2-propanediol + NAD+
-
390589
Pseudomonas putida ML2
propen-1,2-diol + NADH
-
-
-
?
cis-1,2-cyclohexanediol + NAD+
-
390589
Pseudomonas putida
cyclohex-1-ene-1,2-diol + NADH
-
-
-
?
cis-1,2-cyclohexanediol + NAD+
-
390589
Pseudomonas putida ML2
cyclohex-1-ene-1,2-diol + NADH
-
-
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
390589
Pseudomonas putida
catechol + NADH
-
390589
Pseudomonas putida
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
390589
Pseudomonas putida ML2
catechol + NADH
-
390589
Pseudomonas putida ML2
?
glycerol + NAD+
-
390589
Pseudomonas putida
?
-
-
-
?
glycerol + NAD+
-
390589
Pseudomonas putida ML2
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 39000, SDS-PAGE
Pseudomonas putida
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
enzyme assay
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
with NAD+ as cofactor, 100 mM potassium carbonate buffer
Pseudomonas putida
Other publictions for EC 1.3.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739911
de Lima-Morales
Degradation of benzene by Pseu ...
Pseudomonas veronii 1YB2, Pseudomonas veronii 1YdBTEX2, Pseudomonas veronii
Appl. Environ. Microbiol.
82
167-173
2015
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739969
Gutierrez
-
Benzene-induced expression of ...
Rhodococcus sp. 33
Asian J. Microbiol. Biotechnol. Environ. Sci.
17
557-566
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724141
Di Gennaro
Regulated expression systems f ...
Pseudomonas fluorescens, Pseudomonas fluorescens N3
Arch. Microbiol.
195
269-278
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711182
Leneva
Enzymes of naphthalene metabol ...
Pseudomonas fluorescens, Pseudomonas fluorescens 26K
Biochemistry (Moscow)
75
562-569
2010
-
-
-
-
-
-
-
1
-
-
2
2
-
4
-
-
1
-
-
1
2
1
4
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
1
2
1
4
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
711842
Di Gennaro
Development of microbial engin ...
Pseudomonas putida, Pseudomonas putida MST
Ecotoxicol. Environ. Saf.
74
542-549
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654397
Allen
-
Selectivity studies of the ben ...
Pseudomonas putida, Pseudomonas putida ML2
Biocatal. Biotransform.
20
257-264
2002
-
-
-
-
-
-
-
2
-
-
2
3
-
11
-
-
1
-
-
-
1
-
22
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
3
-
-
-
1
-
-
1
-
22
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390589
Fong
Characterization of a novel ci ...
Pseudomonas putida, Pseudomonas putida ML2
FEBS Lett.
451
5-9
1999
-
-
-
-
-
1
-
3
-
1
2
2
-
12
-
-
1
-
-
-
4
1
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
3
-
1
2
2
-
-
-
1
-
-
4
1
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
390588
Fong
Characterization and expressio ...
Pseudomonas putida, Pseudomonas putida ML2
J. Bacteriol.
178
5592-5601
1996
-
-
1
-
-
-
-
-
-
-
1
2
-
18
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390585
Artymiuk
Crystalline cis-benzene glycol ...
Pseudomonas putida, Pseudomonas putida ML2
J. Mol. Biol.
111
203-205
1977
-
-
-
1
-
-
-
-
-
-
1
2
-
12
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390586
Axcell
The metabolism of benzene by b ...
Pseudomonas sp.
Biochem. J.
136
927-934
1973
1
-
-
-
-
2
2
2
-
1
2
1
-
1
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
1
1
-
-
-
1
-
-
1
-
-
2
-
2
-
2
-
1
2
1
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
390587
Gibson
Oxidative degradation of aroma ...
Pseudomonas putida
Biochemistry
7
2653-2662
1968
3
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
3
-
-
1
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
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-
-