Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | reaction rate increases 2fold in the presence of 2-7 mM cysteine | Faecalicatena orotica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | inhibitory above 7 mM | Faecalicatena orotica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
Orotate | - |
Faecalicatena orotica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + NAD+ | Faecalicatena orotica | - |
orotate + NADH + H+ | breakdown of orotate | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Faecalicatena orotica | - |
obligate anaerobic soil bacterium | - |
Purification (Comment) | Organism |
---|---|
partial purification | Faecalicatena orotica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Faecalicatena orotica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + NAD+ | equilibrium favours direction of orotate reduction | Faecalicatena orotica | orotate + NADH + H+ | orotate is identical with 4-carboxyuracil | r | |
dihydroorotate + NAD+ | - |
Faecalicatena orotica | orotate + NADH + H+ | breakdown of orotate | r | |
additional information | not: uracil, cytosine, 5-methylcytosine, thymine as substrates | Faecalicatena orotica | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
pH of assay medium | Faecalicatena orotica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.8 | pH 5.5: 13% of the activity at pH 6.5 | Faecalicatena orotica |
5.5 | 7.8 | pH 7.8: 65% of the activity at pH 6.5 | Faecalicatena orotica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
more stable than at pH 7.0 | Faecalicatena orotica |
7 | - |
less stable than at pH 6.5 | Faecalicatena orotica |