Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not | Faecalicatena orotica | |
glutathione | stimulating effect, but less effective than cysteine | Faecalicatena orotica | |
thioglycolate | stimulating effect, but less effective than cysteine | Faecalicatena orotica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 10% inhibition | Faecalicatena orotica | |
2,4-Dihydroxy-6-methyl pyrimidine | - |
Faecalicatena orotica | |
Acriflavin | partial inhibition | Faecalicatena orotica | |
Barbituric acid | - |
Faecalicatena orotica | |
p-chloromercuribenzoate | completely inhibited by 0.1 mM | Faecalicatena orotica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
Orotate | - |
Faecalicatena orotica | |
0.13 | - |
5-Fluoroorotate | - |
Faecalicatena orotica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + NAD+ | Faecalicatena orotica | enzyme presumably functions mainly in the metabolic synthesis of pyrimidines | orotate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Faecalicatena orotica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
about 90fold purification | Faecalicatena orotica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Faecalicatena orotica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + NAD+ | equilibrium favours direction of orotate reduction | Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
(S)-dihydroorotate + NAD+ | substrate is oxidized by NAD+ and oxygen | Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
5-fluorodihydroorotate + NAD+ | more active substrate for reverse reaction than orotate | Faecalicatena orotica | 5-fluoroorotate + NADH + H+ | - |
r | |
dihydroorotate + NAD+ | enzyme presumably functions mainly in the metabolic synthesis of pyrimidines | Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
additional information | 3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c | Faecalicatena orotica | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values not per enzyme molecule, but per mol of flavin | Faecalicatena orotica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica | |
flavin | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica | |
flavin | interaction of flavin and dihydroorotate is completely dependent on cysteine | Faecalicatena orotica | |
FMN | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica |