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Literature summary for 1.3.1.14 extracted from

  • Friedmann, H.C.; Vennesland, B.
    Purification and properties of diihydroorotic dehydrogenase (1958), J. Biol. Chem., 233, 1398-1406.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not Faecalicatena orotica
glutathione stimulating effect, but less effective than cysteine Faecalicatena orotica
thioglycolate stimulating effect, but less effective than cysteine Faecalicatena orotica

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 10% inhibition Faecalicatena orotica
2,4-Dihydroxy-6-methyl pyrimidine
-
Faecalicatena orotica
Acriflavin partial inhibition Faecalicatena orotica
Barbituric acid
-
Faecalicatena orotica
p-chloromercuribenzoate completely inhibited by 0.1 mM Faecalicatena orotica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
Orotate
-
Faecalicatena orotica
0.13
-
5-Fluoroorotate
-
Faecalicatena orotica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroorotate + NAD+ Faecalicatena orotica enzyme presumably functions mainly in the metabolic synthesis of pyrimidines orotate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Faecalicatena orotica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
about 90fold purification Faecalicatena orotica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Faecalicatena orotica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + NAD+ equilibrium favours direction of orotate reduction Faecalicatena orotica orotate + NADH + H+
-
r
(S)-dihydroorotate + NAD+ substrate is oxidized by NAD+ and oxygen Faecalicatena orotica orotate + NADH + H+
-
r
5-fluorodihydroorotate + NAD+ more active substrate for reverse reaction than orotate Faecalicatena orotica 5-fluoroorotate + NADH + H+
-
r
dihydroorotate + NAD+ enzyme presumably functions mainly in the metabolic synthesis of pyrimidines Faecalicatena orotica orotate + NADH + H+
-
r
additional information 3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c Faecalicatena orotica ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information values not per enzyme molecule, but per mol of flavin Faecalicatena orotica

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
flavin flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
flavin interaction of flavin and dihydroorotate is completely dependent on cysteine Faecalicatena orotica
FMN flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica