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Literature summary for 1.3.1.120 extracted from
- Comparison of two unusual enoyl-CoA reductases in Streptomyces collinus (1993), J. Nat. Prod., 56, 175-185 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
NADPH | pH 7.5, 30°C | Streptomyces collinus |  |
| 0.025 | - |
cyclohex-2-ene-1-carbonyl-CoA | pH 7.5, 30°C | Streptomyces collinus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Streptomyces collinus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxycyclohex-1-ene-1-carbonyl-CoA + NADP+ | Streptomyces collinus | 50% of activity | 3-hydroxycyclohexane-1-carbonyl-CoA + NADPH + H+ | - |
? | |
| cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+ | Streptomyces collinus | 100% of activity, the hydrogen from the pro-4S postion of NADPH is transferred | cyclohexane-1-carbonyl-CoA + NADP+ | - |
r | |
| cyclohex-2-ene-1-carbonyl-CoA + NADP+ | Streptomyces collinus | 100% of activity | cyclohex-1(6),2-diene-1-carbonyl-CoA + NADPH + H+ | - |
r | |
| cyclohexa-1,3-diene-1-carbonyl-CoA + NADP+ | Streptomyces collinus | 28% of activity | cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+ | - |
? | |
| cyclohexa-1,4-diene-1-carbonyl-CoA + NADP+ | Streptomyces collinus | 13% of activity | cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces collinus | P95727 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces collinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxycyclohex-1-ene-1-carbonyl-CoA + NADP+ | 50% of activity | Streptomyces collinus | 3-hydroxycyclohexane-1-carbonyl-CoA + NADPH + H+ | - |
? | |
| cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+ | 100% of activity, the hydrogen from the pro-4S postion of NADPH is transferred | Streptomyces collinus | cyclohexane-1-carbonyl-CoA + NADP+ | - |
r | |
| cyclohex-2-ene-1-carbonyl-CoA + NADP+ | 100% of activity | Streptomyces collinus | cyclohex-1(6),2-diene-1-carbonyl-CoA + NADPH + H+ | - |
r | |
| cyclohexa-1,3-diene-1-carbonyl-CoA + NADP+ | 28% of activity | Streptomyces collinus | cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+ | - |
? | |
| cyclohexa-1,4-diene-1-carbonyl-CoA + NADP+ | 13% of activity | Streptomyces collinus | cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chcA | - |
Streptomyces collinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces collinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces collinus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NAD+, FAD or FMN | Streptomyces collinus | |
| NADP+ | - |
Streptomyces collinus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme catalyzes the final step in the pathwax from shikimic acid to cyclohexanecarboxylic acid | Streptomyces collinus |
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Release 2023.1 (January 2023)
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