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Literature summary for 1.3.1.12 extracted from

  • Lopez-Nieves, S.; Pringle, A.; Hiroshi, A.M.
    Biochemical characterization of TyrA dehydrogenases from Saccharomyces cerevisiae (Ascomycota) and Pleurotus ostreatus (Basidiomycota) (2019), Arch. Biochem. Biophys., 665, 12-19 .
    View publication on PubMed
Search for more literature for 1.3.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharomyces cerevisiae
expressed in Escherichia coli Pleurotus ostreatus

Protein Variants

Protein Variants Comment Organism
N234D the prephenate dehydrogenase activity of the mutant enzyme is 5fold increased compared to the wild type enzyme. L-tyrosine sensitivity is not altered compared to the wild type enzyme. The mutant does not have arognate dehydrogenase activity Pleurotus ostreatus
N234D the prephenate dehydrogenase activity of the mutant enzyme is slightly reduced compared to the wild type enzyme. L-tyrosine sensitivity is not altered compared to the wild type enzyme. The mutant does not have arognate dehydrogenase activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-tyrosine the enzyme is completely inhibited by 0.2 mM L-tyrosine with either NAD+ or NADP+ as cosubstrate Pleurotus ostreatus
L-tyrosine the enzyme exhibits 30% and 60% residual activity at 1 mM L-tyrosine with NAD+ or NADP+ as cosubstrate, respectively Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 prephenate with NAD+ as cosubstrate, at pH 7.6 and 37°C Saccharomyces cerevisiae
0.32
-
 prephenate with NAD+ as cosubstrate, at pH 7.6 and 37°C Pleurotus ostreatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prephenate + NAD+ Saccharomyces cerevisiae
-
 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?
prephenate + NAD+ Pleurotus ostreatus
-
 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus A0A386QX79
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae
-
 Pleurotus ostreatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12000
-
 with NADP+ as cosubstrate, pH 7.6 and 37°C Saccharomyces cerevisiae
30000
-
 with NAD+ as cosubstrate, pH 7.6 and 37°C Saccharomyces cerevisiae
60000
-
 with NADP+ as cosubstrate, pH 7.6 and 37°C Pleurotus ostreatus
300000
-
 with NAD+ as cosubstrate, pH 7.6 and 37°C Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with arogenate Saccharomyces cerevisiae ?
-
 ?
additional information no activity with arogenate Pleurotus ostreatus ?
-
 ?
prephenate + NAD+
-
 Saccharomyces cerevisiae 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?
prephenate + NAD+
-
 Pleurotus ostreatus 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?
prephenate + NADP+ very low activity Saccharomyces cerevisiae 4-hydroxyphenylpyruvate + CO2 + NADPH
-
 ?
prephenate + NADP+ weak activity Pleurotus ostreatus 4-hydroxyphenylpyruvate + CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
PDH
-
 Saccharomyces cerevisiae
PDH
-
 Pleurotus ostreatus
TYR1
-
 Saccharomyces cerevisiae
TYR1
-
 Pleurotus ostreatus
tyrA
-
 Saccharomyces cerevisiae
tyrA
-
 Pleurotus ostreatus
TyrA dehydrogenase
-
 Saccharomyces cerevisiae
TyrA dehydrogenase
-
 Pleurotus ostreatus
TyrAp
-
 Saccharomyces cerevisiae
TyrAp
-
 Pleurotus ostreatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 prephenate with NAD+ as cosubstrate, pH 7.6 and 37°C Saccharomyces cerevisiae
3.09
-
 prephenate with NAD+ as cosubstrate, pH 7.6 and 37°C Pleurotus ostreatus

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor Saccharomyces cerevisiae
NAD+ preferred cofactor Pleurotus ostreatus
NADP+
-
 Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.62
-
 prephenate with NAD+ as cosubstrate, pH 7.6 and 37°C Saccharomyces cerevisiae
204.33
-
 prephenate with NAD+ as cosubstrate, pH 7.6 and 37°C Pleurotus ostreatus