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Literature summary for 1.3.1.12 extracted from

  • Ku, H.; Park, S.; Yang, I.; Kim, S.
    Expression and functional characterization of prephenate dehydrogenase from Streptococcus mutans (2010), Process Biochem., 45, 607-612.
No PubMed abstract available
Search for more literature for 1.3.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
L-tyrosine 0.1 mM, less than 20% residual activity Streptococcus mutans
additional information not inhibitory: NaCl up to 10 mM, EDTA and dithiothreitol up to 1 mM Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 prephenate pH 6.8, 37°C Streptococcus mutans
3.7
-
 NAD+ pH 6.8, 37°C Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41800, calculated, 2 * 41000, SDS-PAGE Streptococcus mutans
41800
-
 2 * 41800, calculated, 2 * 41000, SDS-PAGE Streptococcus mutans
66000
-
 gel filtration Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q8DUW0
-
-
Streptococcus mutans ATCC 700610 Q8DUW0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptococcus mutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
 pH 6.8, 37°C Streptococcus mutans

Storage Stability

Storage Stability Organism
4°C, -20°C, or -80°C, 50 mM Tris, 25% glycerol, no significant differences in activity, even after one month of storage Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prephenate + NAD+
-
 Streptococcus mutans 4-hydroxyphenylpyruvate + NADH + H+ + CO2
-
 ?
prephenate + NAD+
-
 Streptococcus mutans ATCC 700610 4-hydroxyphenylpyruvate + NADH + H+ + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41800, calculated, 2 * 41000, SDS-PAGE Streptococcus mutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptococcus mutans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.4
-
 NAD+ pH 6.8, 37°C Streptococcus mutans
10.5
-
 prephenate pH 6.8, 37°C Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Streptococcus mutans

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 50% of maximum activity Streptococcus mutans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 NAD+ pH 6.8, 37°C Streptococcus mutans
13.9
-
 prephenate pH 6.8, 37°C Streptococcus mutans