Cloned (Comment) | Organism |
---|---|
mutants cloned into pZE21-MCS1 and transformed to Escherichia coli DH5alpha, wild-type and mutants subcloned into vector pET-30XA/LIC and expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | feedback-inhibition-resistant mutants toward L-tyrosine, chorismate mutase activities similar to those of the wild-type, amino acid substitutions either at Tyr263 or at residues 354 to 357 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tyrosine | Tyr263 or residues 354 to 357 of the enzyme are involved in the feedback inhibition | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli K-12 MG1655 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-tagged proteins, to homogeneity by gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.8 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
prephenate + NAD+ | - |
Escherichia coli K-12 MG1655 | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Escherichia coli |
tyrA | - |
Escherichia coli |