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Literature summary for 1.3.1.12 extracted from
- Tyrosine biosynthesis in Aerobacter aerogenes. Purification and properties of chorismate mutase-prephenate dehydrogenase (1970), Biochim. Biophys. Acta, 212, 375-386.
General Stability
| General Stability | Organism |
|---|---|
| stability in Tris-HCl buffer higher than in phosphate buffer | Klebsiella aerogenes |
| tyrosine stabilizes | Klebsiella aerogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Klebsiella aerogenes |  |
| iodoacetate | - |
Klebsiella aerogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | 0.37 | prephenate | - |
Klebsiella aerogenes | |
| 0.6 | - |
NAD+ | - |
Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | 81000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel filtration, gel electrophoresis | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate + NAD+ | Klebsiella aerogenes | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, for at least 10 days | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate + NAD+ | - |
Klebsiella aerogenes | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
| prephenate + NAD+ | biosynthesis of L-tyrosine | Klebsiella aerogenes | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Klebsiella aerogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dithiothreitol, protection against thermal inactivation | Klebsiella aerogenes |
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Release 2023.1 (January 2023)
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