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Literature summary for 1.3.1.118 extracted from

  • Quemard, A.; Sacchettini, J.; Dessen, A.; Vilcheze, C.; Bittman, R.; Jacobs, W.J.; Blanchard, J.
    Enzymatic characterization of the target for isoniazid in Mycobacterium tuberculosis (1995), Biochemistry, 34, 8235-8241 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
S94A mutation confers resistance to both isoniazid and ethionamide. Binding of NADH to the mutant is altered Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
NAD+ linear competitive inhibitor versus NADH Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
trans-2-hexadecenoyl-CoA pH 6.8, 25°C, wild-type enzyme Mycobacterium tuberculosis
0.002
-
trans-2-octenoyl-[acyl-carrier protein] pH 6.8, 25°C, wild-type enzyme Mycobacterium tuberculosis
0.0027
-
trans-2-octenoyl-[acyl-carrier protein] pH 6.8, 25°C, mutant enzyme S94A Mycobacterium tuberculosis
0.0045
-
NADH pH 6.8, 25°C, cosubstrate: trans-2-hexadecenoyl-CoA, wild-type enzyme Mycobacterium tuberculosis
0.0058
-
NADH pH 6.8, 25°C, cosubstrate: trans-2-dodecenoyl-CoA, wild-type enzyme Mycobacterium tuberculosis
0.0076
-
NADH pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-CoA, wild-type enzyme Mycobacterium tuberculosis
0.0081
-
NADH pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-[acyl-carrier protein], wild-type enzyme Mycobacterium tuberculosis
0.0373
-
NADH pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-[acyl-carrier protein], mutant enzyme S94A Mycobacterium tuberculosis
0.048
-
trans-2-dodecenoyl-CoA pH 6.8, 25°C, wild-type enzyme Mycobacterium tuberculosis
0.065
-
NADH pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-CoA, mutant enzyme S94A Mycobacterium tuberculosis
0.467
-
trans-2-octenoyl-CoA pH 6.8, 25°C, wild-type enzyme Mycobacterium tuberculosis
0.688
-
trans-2-octenoyl-CoA pH 6.8, 25°C, mutant enzyme S94A Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WGR1 cf. EC 1.3.1.9
-
Mycobacterium tuberculosis ATCC 25618 P9WGR1 cf. EC 1.3.1.9
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with crotonoyl-CoA. The enzyme preferentially reduces long-chain 2-trans-enoyl-ACP substrates (12-24 carbons). The two substrates bind to InhA via a sequential kinetic mechanism, with the preferred ordered addition of NADH and the enoyl substrate. The chemical mechanism involves stereospecific hydride transfer of the 4S hydrogen of NADH to the C3 position of the 2-trans-enoyl substrate, followed by protonation at C2 of an enzymestabilized enolate intermediate Mycobacterium tuberculosis ?
-
?
additional information no activity with crotonoyl-CoA. The enzyme preferentially reduces long-chain 2-trans-enoyl-ACP substrates (12-24 carbons). The two substrates bind to InhA via a sequential kinetic mechanism, with the preferred ordered addition of NADH and the enoyl substrate. The chemical mechanism involves stereospecific hydride transfer of the 4S hydrogen of NADH to the C3 position of the 2-trans-enoyl substrate, followed by protonation at C2 of an enzymestabilized enolate intermediate Mycobacterium tuberculosis ATCC 25618 ?
-
?
trans-2-dodecenoyl-CoA + NADH + H+
-
Mycobacterium tuberculosis dodecanoyl-CoA + NAD+
-
?
trans-2-dodecenoyl-CoA + NADH + H+
-
Mycobacterium tuberculosis ATCC 25618 dodecanoyl-CoA + NAD+
-
?
trans-2-hexadecenoyl-CoA + NADH + H+
-
Mycobacterium tuberculosis hexadecanoyl-CoA + NAD+
-
?
trans-2-hexadecenoyl-CoA + NADH + H+
-
Mycobacterium tuberculosis ATCC 25618 hexadecanoyl-CoA + NAD+
-
?
trans-2-octenoyl-CoA + NADH + H+
-
Mycobacterium tuberculosis octanoyl-CoA + NAD+
-
?
trans-2-octenoyl-CoA + NADH + H+
-
Mycobacterium tuberculosis ATCC 25618 octanoyl-CoA + NAD+
-
?
trans-2-octenoyl-[acyl-carrier protein] + NADH + H+
-
Mycobacterium tuberculosis octanoyl-[acyl-carrier protein] + NAD+
-
?
trans-2-octenoyl-[acyl-carrier protein] + NADH + H+
-
Mycobacterium tuberculosis ATCC 25618 octanoyl-[acyl-carrier protein] + NAD+
-
?

Subunits

Subunits Comment Organism
homodimer
-
Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
InhA Protein
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism the enzyme is involved in mycolic acid biosynthesis Mycobacterium tuberculosis