Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
S94A | mutation confers resistance to both isoniazid and ethionamide. Binding of NADH to the mutant is altered | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | linear competitive inhibitor versus NADH | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
trans-2-hexadecenoyl-CoA | pH 6.8, 25°C, wild-type enzyme | Mycobacterium tuberculosis | |
0.002 | - |
trans-2-octenoyl-[acyl-carrier protein] | pH 6.8, 25°C, wild-type enzyme | Mycobacterium tuberculosis | |
0.0027 | - |
trans-2-octenoyl-[acyl-carrier protein] | pH 6.8, 25°C, mutant enzyme S94A | Mycobacterium tuberculosis | |
0.0045 | - |
NADH | pH 6.8, 25°C, cosubstrate: trans-2-hexadecenoyl-CoA, wild-type enzyme | Mycobacterium tuberculosis | |
0.0058 | - |
NADH | pH 6.8, 25°C, cosubstrate: trans-2-dodecenoyl-CoA, wild-type enzyme | Mycobacterium tuberculosis | |
0.0076 | - |
NADH | pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-CoA, wild-type enzyme | Mycobacterium tuberculosis | |
0.0081 | - |
NADH | pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-[acyl-carrier protein], wild-type enzyme | Mycobacterium tuberculosis | |
0.0373 | - |
NADH | pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-[acyl-carrier protein], mutant enzyme S94A | Mycobacterium tuberculosis | |
0.048 | - |
trans-2-dodecenoyl-CoA | pH 6.8, 25°C, wild-type enzyme | Mycobacterium tuberculosis | |
0.065 | - |
NADH | pH 6.8, 25°C, cosubstrate: trans-2-octenoyl-CoA, mutant enzyme S94A | Mycobacterium tuberculosis | |
0.467 | - |
trans-2-octenoyl-CoA | pH 6.8, 25°C, wild-type enzyme | Mycobacterium tuberculosis | |
0.688 | - |
trans-2-octenoyl-CoA | pH 6.8, 25°C, mutant enzyme S94A | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28500 | - |
SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WGR1 | cf. EC 1.3.1.9 | - |
Mycobacterium tuberculosis ATCC 25618 | P9WGR1 | cf. EC 1.3.1.9 | - |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with crotonoyl-CoA. The enzyme preferentially reduces long-chain 2-trans-enoyl-ACP substrates (12-24 carbons). The two substrates bind to InhA via a sequential kinetic mechanism, with the preferred ordered addition of NADH and the enoyl substrate. The chemical mechanism involves stereospecific hydride transfer of the 4S hydrogen of NADH to the C3 position of the 2-trans-enoyl substrate, followed by protonation at C2 of an enzymestabilized enolate intermediate | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with crotonoyl-CoA. The enzyme preferentially reduces long-chain 2-trans-enoyl-ACP substrates (12-24 carbons). The two substrates bind to InhA via a sequential kinetic mechanism, with the preferred ordered addition of NADH and the enoyl substrate. The chemical mechanism involves stereospecific hydride transfer of the 4S hydrogen of NADH to the C3 position of the 2-trans-enoyl substrate, followed by protonation at C2 of an enzymestabilized enolate intermediate | Mycobacterium tuberculosis ATCC 25618 | ? | - |
? | |
trans-2-dodecenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis | dodecanoyl-CoA + NAD+ | - |
? | |
trans-2-dodecenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | dodecanoyl-CoA + NAD+ | - |
? | |
trans-2-hexadecenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis | hexadecanoyl-CoA + NAD+ | - |
? | |
trans-2-hexadecenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | hexadecanoyl-CoA + NAD+ | - |
? | |
trans-2-octenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis | octanoyl-CoA + NAD+ | - |
? | |
trans-2-octenoyl-CoA + NADH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | octanoyl-CoA + NAD+ | - |
? | |
trans-2-octenoyl-[acyl-carrier protein] + NADH + H+ | - |
Mycobacterium tuberculosis | octanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
trans-2-octenoyl-[acyl-carrier protein] + NADH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | octanoyl-[acyl-carrier protein] + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
InhA Protein | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in mycolic acid biosynthesis | Mycobacterium tuberculosis |