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Literature summary for 1.3.1.116 extracted from
- Characterization of the baiH gene encoding a bile acid-inducible NADH flavin oxidoreductase from Eubacterium sp. strain VPI 12708 (1993), J. Bacteriol., 175, 3002-3012 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
[Clostridium] scindens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 2.0 mM, 42% inhibition | [Clostridium] scindens |  |
| Acriflavine | 0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity | [Clostridium] scindens | |
| Cu2+ | 1 mM, 1% residual activity | [Clostridium] scindens | |
| CuCl2 | 1.0 mM, 99% inhibition | [Clostridium] scindens | |
| EDTA | 5.0 mM, 48% inhibition; 5 mM, 52% residual activity | [Clostridium] scindens | |
| HgCl2 | 1 mM, 97% inhibition | [Clostridium] scindens | |
| iodoacetate | 1.0 mM, 9% inhibition | [Clostridium] scindens | |
| N-bromosuccinimide | 0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition | [Clostridium] scindens | |
| N-ethylmaleimide | 1.0 mM, 96% inhibition; 1 mM, 4% residual activity | [Clostridium] scindens | |
| o-phenanthroline | 5 mM, 58% residual activity | [Clostridium] scindens | |
| p-chloromercuriphenyl sulfonate | 0.2 mM, 5% residual activity | [Clostridium] scindens | |
| p-chloromercuriphenyl sulfonic acid | 0.2 mM, 95% inhibition | [Clostridium] scindens | |
| Zn2+ | 1 mM, 27% residual activity | [Clostridium] scindens | |
| ZnCl2 | 1.0 mM, 73% inhibition | [Clostridium] scindens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
SDS-PAGE | [Clostridium] scindens |
| 210000 | - |
gel filtration | [Clostridium] scindens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Clostridium] scindens | P32370 | - |
- |
| [Clostridium] scindens JCM 10418 | P32370 | - |
- |
| [Clostridium] scindens VPI 12708 | P32370 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Clostridium] scindens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 48.36 | - |
pH 6.8, 20°C | [Clostridium] scindens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAD + NADH + H+ | - |
[Clostridium] scindens | FADH2 + NAD+ | - |
? | |
| FAD + NADH + H+ | - |
[Clostridium] scindens JCM 10418 | FADH2 + NAD+ | - |
? | |
| FAD + NADH + H+ | - |
[Clostridium] scindens VPI 12708 | FADH2 + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 72000, SDS-PAGE | [Clostridium] scindens |
| trimer | 3 * 72000, SDS-PAGE, x * 72006, calculated from sequence | [Clostridium] scindens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BaiH | - |
[Clostridium] scindens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | [Clostridium] scindens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | [Clostridium] scindens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
[Clostridium] scindens | |
| NADH | - |
[Clostridium] scindens | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| [Clostridium] scindens | inducible by cholate | up |
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