Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.116 extracted from

  • Baron, S.; Hylemon, P.
    Expression of the bile acid-inducible NADH flavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli (1995), Biochim. Biophys. Acta, 1249, 145-154 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli [Clostridium] scindens
subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid [Clostridium] scindens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
[Clostridium] scindens
Acriflavine
-
[Clostridium] scindens
CuCl2
-
[Clostridium] scindens
EDTA
-
[Clostridium] scindens
EGTA
-
[Clostridium] scindens
HgCl2
-
[Clostridium] scindens
additional information iodoacetate and iodoacetamide show no inhibition [Clostridium] scindens
N-bromosuccinimide
-
[Clostridium] scindens
N-ethylmaleimide
-
[Clostridium] scindens
p-chloromercuriphenylsulfonic acid
-
[Clostridium] scindens
Sodium azide
-
[Clostridium] scindens
ZnCl2
-
[Clostridium] scindens

Metals/Ions

Metals/Ions Comment Organism Structure
copper 1 mol of copper per mol of subunit [Clostridium] scindens
copper contains 1 mol of copper per subunit [Clostridium] scindens
Iron 2 mol of iron per mol of subunit [Clostridium] scindens
Iron contains up to 2 mol of iron per subunit [Clostridium] scindens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71400
-
SDS-PAGE [Clostridium] scindens
210000
-
gel filtration [Clostridium] scindens
210000
-
non-denaturing PAGE [Clostridium] scindens

Organism

Organism UniProt Comment Textmining
[Clostridium] scindens P32370
-
-
[Clostridium] scindens JCM 10418 P32370
-
-
[Clostridium] scindens VPI 12708 P32370
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Clostridium] scindens
recombinant protein [Clostridium] scindens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones [Clostridium] scindens ?
-
?
additional information the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones [Clostridium] scindens JCM 10418 ?
-
?
additional information the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones [Clostridium] scindens VPI 12708 ?
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 71400, SDS-PAGE [Clostridium] scindens
trimer 3 * 72000, SDS-PAGE [Clostridium] scindens

Synonyms

Synonyms Comment Organism
BaiH
-
[Clostridium] scindens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
[Clostridium] scindens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
[Clostridium] scindens

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol of FAD per mol of subunit [Clostridium] scindens
FAD contains 1 mol of FAD [Clostridium] scindens
NADH
-
[Clostridium] scindens

pI Value

Organism Comment pI Value Maximum pI Value
[Clostridium] scindens isoelectric focusing
-
6

General Information

General Information Comment Organism
physiological function enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation [Clostridium] scindens