Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.115 extracted from

  • Kang, D.; Ridlon, J.; Moore II, D.; Barnes, S.; Hylemon, P.
    Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductases (2008), Biochim. Biophys. Acta, 1781, 16-25 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.115

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli [Clostridium] scindens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418
-
 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418
-
 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Clostridium] scindens
-
-
-
[Clostridium] scindens P19410
-
-
[Clostridium] scindens JCM 10418
-
-
-
[Clostridium] scindens VPI 12708 P19410
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Clostridium] scindens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens JCM 10418 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens VPI 12708 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens VPI 12708 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
 [Clostridium] scindens JCM 10418 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens VPI 12708 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomer [Clostridium] scindens VPI 12708 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE [Clostridium] scindens

Synonyms

Synonyms Comment Organism
3-oxo-DELTA4-cholenoic acid oxidoreductase
-
 [Clostridium] scindens
BaiCD
-
 [Clostridium] scindens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at [Clostridium] scindens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at [Clostridium] scindens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 [Clostridium] scindens

General Information

General Information Comment Organism
metabolism the enzyme participates in the bile acid 7alpha-dehydroxylation pathway [Clostridium] scindens