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Literature summary for 1.3.1.109 extracted from

  • Li, F.; Hinderberger, J.; Seedorf, H.; Zhang, J.; Buckel, W.; Thauer, R.K.
    Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri (2008), J. Bacteriol., 190, 843-850.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.109

General Stability

General Stability Organism
FMN can not substitute for FAD in stabilizing the enzyme activity Clostridium kluyveri

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Clostridium kluyveri 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri
36000
-
 x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri
41000
-
 x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri
320000
-
 gel filtration Clostridium kluyveri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster Clostridium kluyveri
-
 butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium kluyveri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium kluyveri

Storage Stability

Storage Stability Organism
4°C, 2-3 days, in the presence of FAD and under anoxic conditions the butyryl-CoA dehydrogenase/Etf complex loses 50% of its activity Clostridium kluyveri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 Clostridium kluyveri butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster the enzyme couples the endergonic reduction of ferredoxin with NADH to the exergonic reduction of crotonyl-CoA to butanoyl-CoA Clostridium kluyveri butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?

Subunits

Subunits Comment Organism
? x * 41000 + x * 36000 + x * 28000, during purification the butanoyl-CoA dehydrogenase/Etf complex partially dissociated into its components, SDS-PAGE Clostridium kluyveri

Synonyms

Synonyms Comment Organism
butyryl-CoA dehydrogenase/Etf complex
-
 Clostridium kluyveri