Application | Comment | Organism |
---|---|---|
synthesis | construction of a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e. (S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides | Burkholderia sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Burkholderia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | Q59I44 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloroacrylate + NADPH + H+ | no activity with NADH | Burkholderia sp. | (S)-2-chloropropanoate + NADP+ | - |
? |