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Literature summary for 1.3.1.100 extracted from

  • Wallwey, C.; Matuschek, M.; Xie, X.; Li, S.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus: Conversion of chanoclavine-I aldehyde to festuclavine by the festuclavine synthase FgaFS in the presence of the old yellow enzyme FgaOx3 (2010), Org. Biomol. Chem., 8, 3500-3508.
    View publication on PubMed
Search for more literature for 1.3.1.100

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1 Blue MRF’ cells Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 45000, SDS-PAGE Aspergillus fumigatus
46000
-
 gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus V5IVA1
-
-
Aspergillus fumigatus B5233 V5IVA1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chanoclavine-I aldehyde + NADH + H+ in the presence of FgaFS only Aspergillus fumigatus dihydrochanoclavine-I aldehyde + NAD+
-
 ?
chanoclavine-I aldehyde + NADH + H+ in the presence of FgaFS only Aspergillus fumigatus B5233 dihydrochanoclavine-I aldehyde + NAD+
-
 ?
additional information the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH Aspergillus fumigatus ?
-
 ?
additional information the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH Aspergillus fumigatus B5233 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
FgaOx3
-
 Aspergillus fumigatus
old yellow enzyme
-
 Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Aspergillus fumigatus
NADH
-
 Aspergillus fumigatus