Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
A95V | the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme | Staphylococcus aureus |
F204S | inactive, the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme | Staphylococcus aureus |
I193S | the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme | Staphylococcus aureus |
K41N | strongly decreased activity for NADPH and increased activity with NADH | Staphylococcus aureus |
R40Q | strongly decreased activity for NADPH and increased activity with NADH | Staphylococcus aureus |
R40Q/K41N | strongly decreased activity for NADPH and increased activity with NADH | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-chloro-2-phenoxyphenol | - |
Staphylococcus aureus | |
5-ethyl-2-phenoxyphenol | - |
Staphylococcus aureus | |
triclosan | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.008 | - |
Crotonyl-N-acetyl-cysteamine | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.0114 | - |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] | mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.0115 | - |
crotonyl-[Staphylococcus aureus acyl carrier protein] | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.0184 | - |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] | mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.0233 | - |
dodecenoyl-N-acetyl-cysteamine | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.0241 | - |
dodec-2-enoyl-CoA | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.2694 | - |
NADPH | mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
0.4296 | - |
NADPH | mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
1 | - |
NADPH | Km above 1 mM, mutant enzyme I193S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA resin chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-N-acetyl-cysteamine + NADPH + H+ | - |
Staphylococcus aureus | butyryl-N-acetyl-cysteamine + NADP+ | - |
? | |
crotonyl-[Staphylococcus aureus acyl carrier protein] + NADPH + H+ | - |
Staphylococcus aureus | butyryl-[Staphylococcus aureus acyl carrier protein] + NADP+ | - |
? | |
dodec-2-enoyl-CoA + NADPH + H+ | - |
Staphylococcus aureus | dodecanoyl-CoA + NADP+ | - |
r | |
dodecenoyl-CoA + NADPH + H+ | - |
Staphylococcus aureus | dodecanoyl-CoA + NADP+ | - |
? | |
dodecenoyl-N-acetyl-cysteamine + NADPH + H+ | - |
Staphylococcus aureus | dodecanoyl-N-acetyl-cysteamine + NADP+ | - |
? | |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] + NADPH + H+ | - |
Staphylococcus aureus | dodecanoyl-[Staphylococcus aureus acyl carrier protein] + NADP+ | - |
? | |
additional information | no activity with crotonyl-CoA and NADH | Staphylococcus aureus | ? | - |
? | |
trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+ | prefers acyl carrier protein substrates carrying fatty acids with long acyl chains | Staphylococcus aureus | acyl-[acyl-carrier protein] + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl reductase | - |
Staphylococcus aureus |
FabI | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
Crotonyl-N-acetyl-cysteamine | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
11.6 | - |
crotonyl-[Staphylococcus aureus acyl carrier protein] | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
18 | - |
dodec-2-enoyl-CoA | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
18.4 | - |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] | mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
30.4 | - |
dodecenoyl-N-acetyl-cysteamine | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
130.2 | - |
NADPH | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
130.2 | - |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] | wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus | |
157.3 | - |
dodecenoyl-[Staphylococcus aureus acyl carrier protein] | mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent | Staphylococcus aureus |