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Literature summary for 1.3.1.10 extracted from
- Enoyl acyl carrier protein reductase from Escherichia coli (1969), Methods Enzymol., 14, 66-73.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | - |
Escherichia coli |  |
| p-hydroxymercuribenzoate | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| no separation from NADH specific enzyme | Escherichia coli |
| sonification and chromatography using DEAE-Cellulose, Sephadex G-100, Hydroxylapatite | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.31 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM phosphate buffer, pH 6.5, 5 mM 2-mercaptoethanol, inactivation in 2-3 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hexenoyl-[acyl-carrier protein] + NADPH | - |
Escherichia coli | hexanoyl-[acyl-carrier protein] + NADP+ | - |
? | |
| crotonyl-[acyl-carrier protein] + NADPH | - |
Escherichia coli | butyryl-[acyl-carrier protein] + NADP+ | - |
? | |
| additional information | active on substrates with acyl chain length C4-C16 | Escherichia coli | ? | - |
? | |
| additional information | inactive with enoyl-CoA substrates | Escherichia coli | ? | - |
? | |
| additional information | part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters | Escherichia coli | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min stable | Escherichia coli |
| 60 | - |
5 min, inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
no measurements below pH 6 carried out | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
inactive above pH 8, no measurement below pH 6 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
unstable at | Escherichia coli |
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