Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
thylakoid membrane | - |
Mantoniella squamata | 42651 | - |
thylakoid membrane | - |
Spinacia oleracea | 42651 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
antheraxanthin + L-ascorbate | Mantoniella squamata | - |
zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
antheraxanthin + L-ascorbate | Spinacia oleracea | - |
zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
antheraxanthin + L-ascorbate | Mantoniella squamata CCAP 1965/1 | - |
zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
additional information | Mantoniella squamata | high concentrations of available violaxanthin, as found in enzyme assays with pure violaxanthin, lead to saturation of the VDE and a strong competition with the intermediate reaction product Ax, thus decreasing the ratio of the second deepoxidation rate to the first de-epoxidation rate | ? | - |
? | |
additional information | Spinacia oleracea | spinach VDE is able to de-epoxidize violaxanthin bound to spinach or Mantoniella squamata light harvesting complexes in a comparable manner, rate constants for first and second reaction step, overview | ? | - |
? | |
additional information | Mantoniella squamata CCAP 1965/1 | high concentrations of available violaxanthin, as found in enzyme assays with pure violaxanthin, lead to saturation of the VDE and a strong competition with the intermediate reaction product Ax, thus decreasing the ratio of the second deepoxidation rate to the first de-epoxidation rate | ? | - |
? | |
violaxanthin + L-ascorbate | Mantoniella squamata | - |
antheraxanthin + L-dehydroascorbate + H2O | - |
? | |
violaxanthin + L-ascorbate | Spinacia oleracea | - |
antheraxanthin + L-dehydroascorbate + H2O | - |
? | |
violaxanthin + L-ascorbate | Mantoniella squamata CCAP 1965/1 | - |
antheraxanthin + L-dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mantoniella squamata | - |
- |
- |
Mantoniella squamata CCAP 1965/1 | - |
- |
- |
Spinacia oleracea | Q9SM43 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by preparation of thylakoid membranes, further purifications of the VDE by, i.e. anion exchange chromatography and gel filtration, lead to a significant loss of VDE activity | Mantoniella squamata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
antheraxanthin + L-ascorbate | - |
Mantoniella squamata | zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
antheraxanthin + L-ascorbate | - |
Spinacia oleracea | zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
antheraxanthin + L-ascorbate | - |
Mantoniella squamata CCAP 1965/1 | zeaxanthin + L-dehydroascorbate + H2O | - |
? | |
additional information | high concentrations of available violaxanthin, as found in enzyme assays with pure violaxanthin, lead to saturation of the VDE and a strong competition with the intermediate reaction product Ax, thus decreasing the ratio of the second deepoxidation rate to the first de-epoxidation rate | Mantoniella squamata | ? | - |
? | |
additional information | spinach VDE is able to de-epoxidize violaxanthin bound to spinach or Mantoniella squamata light harvesting complexes in a comparable manner, rate constants for first and second reaction step, overview | Spinacia oleracea | ? | - |
? | |
additional information | Mantoniella squamata VDE exhibits a very low ratio of the second de-epoxidation rate to the first de-epoxidation rate in thylakoids or in enzyme assays with thte purified light-harvesting complex. The interaction between the isolated light-harvesting complex and the VDE can influence the ratio of the two de-epoxidation rates. Mantoniella squamata VDE is able to de-epoxidize violaxanthin bound to spinach light harvesting complex II better than bound to Mantoniella squamata light harvesting complex II, the latter shows accumulation of intermediate antheraxanthin in the membranes, rate constants for first and second reaction step, overview | Mantoniella squamata | ? | - |
? | |
additional information | high concentrations of available violaxanthin, as found in enzyme assays with pure violaxanthin, lead to saturation of the VDE and a strong competition with the intermediate reaction product Ax, thus decreasing the ratio of the second deepoxidation rate to the first de-epoxidation rate | Mantoniella squamata CCAP 1965/1 | ? | - |
? | |
additional information | Mantoniella squamata VDE exhibits a very low ratio of the second de-epoxidation rate to the first de-epoxidation rate in thylakoids or in enzyme assays with thte purified light-harvesting complex. The interaction between the isolated light-harvesting complex and the VDE can influence the ratio of the two de-epoxidation rates. Mantoniella squamata VDE is able to de-epoxidize violaxanthin bound to spinach light harvesting complex II better than bound to Mantoniella squamata light harvesting complex II, the latter shows accumulation of intermediate antheraxanthin in the membranes, rate constants for first and second reaction step, overview | Mantoniella squamata CCAP 1965/1 | ? | - |
? | |
violaxanthin + L-ascorbate | - |
Mantoniella squamata | antheraxanthin + L-dehydroascorbate + H2O | - |
? | |
violaxanthin + L-ascorbate | - |
Spinacia oleracea | antheraxanthin + L-dehydroascorbate + H2O | - |
? | |
violaxanthin + L-ascorbate | - |
Mantoniella squamata CCAP 1965/1 | antheraxanthin + L-dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VDE | - |
Mantoniella squamata |
VDE | - |
Spinacia oleracea |
Vx de-epoxidase | - |
Mantoniella squamata |
Vx de-epoxidase | - |
Spinacia oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mantoniella squamata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
assay at | Mantoniella squamata |