Cloned (Comment) | Organism |
---|---|
the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis | Linum usitatissimum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-lariciresinol + NADPH + H+ | Linum usitatissimum | - |
(+)-secoisolariciresinol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Linum usitatissimum | Q4R0H9 | cv. Barbara | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no enzyme activity is detected in the inner integument and the embryo regardless of the developmental stage | Linum usitatissimum | - |
parenchymal cell | cell layer-specific localization of secoisolariciresinol diglucoside (SDG) in flaxseed coats | Linum usitatissimum | - |
seed | spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview | Linum usitatissimum | - |
seed coat | the parenchymatous cell layer of the outer integument of flaxseed coats, the enzyme is strongly expressed in the seed coats of developing flaxseed, quantitative RT-PCR expression analysis or recombinant PLR1 promoter construct in transgenic flaxseed | Linum usitatissimum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-lariciresinol + NADPH + H+ | - |
Linum usitatissimum | (+)-secoisolariciresinol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional pinoresinol-lariciresinol reductase | UniProt | Linum usitatissimum |
More | cf. EC 1.23.1.3 | Linum usitatissimum |
pinoresinol-lariciresinol reductase | - |
Linum usitatissimum |
PLR-Lu1 | UniProt | Linum usitatissimum |
PLR1 | - |
Linum usitatissimum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Linum usitatissimum |
General Information | Comment | Organism |
---|---|---|
metabolism | the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, proposed biosynthesis pathway of SDG oligomer, overview | Linum usitatissimum |
physiological function | the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, responsible for the synthesis of the enantiomer (+)-secoisolariciresinol, spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview | Linum usitatissimum |