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Literature summary for 1.23.1.4 extracted from

  • Fang, J.; Ramsay, A.; Renouard, S.; Hano, C.; Lamblin, F.; Chabbert, B.; Mesnard, F.; Schneider, B.
    Laser microdissection and spatiotemporal pinoresinol-lariciresinol reductase gene expression assign the cell layer-specific accumulation of secoisolariciresinol diglucoside in flaxseed coats (2016), Front. Plant Sci., 7, 1743 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis Linum usitatissimum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-lariciresinol + NADPH + H+ Linum usitatissimum
-
(+)-secoisolariciresinol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Linum usitatissimum Q4R0H9 cv. Barbara
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information no enzyme activity is detected in the inner integument and the embryo regardless of the developmental stage Linum usitatissimum
-
parenchymal cell cell layer-specific localization of secoisolariciresinol diglucoside (SDG) in flaxseed coats Linum usitatissimum
-
seed spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview Linum usitatissimum
-
seed coat the parenchymatous cell layer of the outer integument of flaxseed coats, the enzyme is strongly expressed in the seed coats of developing flaxseed, quantitative RT-PCR expression analysis or recombinant PLR1 promoter construct in transgenic flaxseed Linum usitatissimum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-lariciresinol + NADPH + H+
-
Linum usitatissimum (+)-secoisolariciresinol + NADP+
-
?

Synonyms

Synonyms Comment Organism
bifunctional pinoresinol-lariciresinol reductase UniProt Linum usitatissimum
More cf. EC 1.23.1.3 Linum usitatissimum
pinoresinol-lariciresinol reductase
-
Linum usitatissimum
PLR-Lu1 UniProt Linum usitatissimum
PLR1
-
Linum usitatissimum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Linum usitatissimum

General Information

General Information Comment Organism
metabolism the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, proposed biosynthesis pathway of SDG oligomer, overview Linum usitatissimum
physiological function the pinoresinol-lariciresinol reductase gene, LuPLR1, encoding bifunctional pinoresinol-lariciresinol reductase, is a key gene involved in secoisolariciresinol diglucoside (SDG) biosynthesis, responsible for the synthesis of the enantiomer (+)-secoisolariciresinol, spatiotemporal regulation of LuPLR1 gene expression in flaxseed, overview Linum usitatissimum