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Literature summary for 1.23.1.2 extracted from

  • Dinkova-Kostova, A.T.; Gang, D.R.; Davin, L.B.; Bedgar, D.L.; Chu, A.; Lewis, N.G.
    (+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia. Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase (1996), J. Biol. Chem., 271, 29473-29482.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Nova-Blue cells Forsythia x intermedia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39400
-
x * 39400, calculated from amino acid sequence Forsythia x intermedia
59000
-
gel filtration Forsythia x intermedia

Organism

Organism UniProt Comment Textmining
Forsythia x intermedia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Affinity Blue Gel filtration, phenyl Sepharose column chromatography, hydroxyapatite column chromatography, 2’,5’ ADP-Sepharose column chromatography, Affinity-Yellow gel filtration, and Superose 12 gel filtration Forsythia x intermedia

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Forsythia x intermedia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000083
-
crude extract, at pH 7.4 and 30°C Forsythia x intermedia
0.255
-
after 3060fold purification, at pH 7.4 and 30°C Forsythia x intermedia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-lariciresinol + NADPH + H+
-
Forsythia x intermedia (-)-secoisolariciresinol + NADP+
-
?

Subunits

Subunits Comment Organism
monomer or dimer x * 35000-36000, SDS-PAGE Forsythia x intermedia
monomer or dimer x * 39400, calculated from amino acid sequence Forsythia x intermedia

Synonyms

Synonyms Comment Organism
(+)-pinoresinol/(+)-lariciresinol reductase
-
Forsythia x intermedia
PLR
-
Forsythia x intermedia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Forsythia x intermedia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Forsythia x intermedia

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Forsythia x intermedia

pI Value

Organism Comment pI Value Maximum pI Value
Forsythia x intermedia isoelectric focusing
-
5.7
Forsythia x intermedia calculated from amino acid sequence
-
7.1

General Information

General Information Comment Organism
metabolism (+)-pinoresinol/(+)-lariciresinol reductase is a pivotal branchpoint enzyme in lignan biosynthesis Forsythia x intermedia