Literature summary for 1.21.99.5 extracted from

Reinhold, A.; Westermann, M.; Seifert, J.; von Bergen, M.; Schubert, T.; Diekert, G.
Impact of vitamin B12 on formation of the tetrachloroethene reductive dehalogenase in Desulfitobacterium hafniense strain Y51 (2012), Appl. Environ. Microbiol., 78, 8025-8032.

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Activating Compound

Activating Compound	Comment	Organism	Structure
vitamin B12	tetrachloroethene dehalogenase formation in fumarate-grown cells is influenced by vitamin B12	Desulfitobacterium hafniense

Organism

Organism	UniProt	Comment	Textmining
Desulfitobacterium hafniense	-	-	-
Desulfitobacterium hafniense Y51	-	-	-

Expression

Organism	Comment	Expression
Desulfitobacterium hafniense	tetrachloroethene-depleted cells grown for several subcultivation steps on fumarate as an alternative electron acceptor lose the tetrachloroethene-reductive dehalogenase PceA activity by the transposition of the pce gene cluster. In the absence of vitamin B12, a gradual decrease of the PceA activity and protein amount is observed. In the presence of vitamin B12, a significant delay in the decrease of the PceA activity with an more than 90% loss after 20 subcultivation steps is observed. This corresponds to the decrease in the pceA gene level. In the absence or presence of exogenous vitamin B12, the intracellular corrinoid level decreases in fumarate-grown cells and the PceA precursor forms catalytically inactive, corrinoid-free multiprotein aggregates	additional information

General Information

General Information	Comment	Organism
metabolism	according to a model for the maturation of PceA, the pce genes are transcribed in tetrachloroethene-grown cells and the PceA cofactor-free precursor is formed and binds to the PceT chaperone. When corrinoid cofactor is provided by de novo biosynthesis, it is incorporated into prePceA. After incorporation of this cofactor and assembly and incorporation of the iron-sulfur clusters, the precursor protein is correctly folded and exported to the exoplasm by the Tat machinery. After cleavage of the signal peptide, the protein is bound to PceB, which may serve as a membrane anchor for PceA. In cells subcultivated for a few steps with fumarate instead of tetrachloroethene, corrinoid biosynthesis is impeded. This causes aggregation of the prePceA together with PceT and other proteins inside the cells. Excision of the pce gene cluster occurs upon longterm cultivation with fumarate. The loss of the gene cluster is delayed in the presence of exogenous vitamin B12	Desulfitobacterium hafniense

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Release 2023.1 (January 2023)