Cloned (Comment) | Organism |
---|---|
expression of pceA gene in Escherichia coli BL21 | Sulfurospirillum multivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Sulfurospirillum multivorans | 5737 | - |
cytoplasmic membrane | - |
Sulfurospirillum multivorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | cobalamin-containing enzyme | Sulfurospirillum multivorans | |
Cobalt | - |
Sulfurospirillum multivorans | |
Fe2+ | two Fe/S clusters | Sulfurospirillum multivorans | |
Fe2+ | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
Iron-sulfur cluster | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
iron-sulfur clusters | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
S | two Fe/S clusters | Sulfurospirillum multivorans | |
S | two Fe4-S4 clusters | Sulfurospirillum multivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55890 | - |
amino acid sequence analysis | Sulfurospirillum multivorans |
57000 | - |
processed enzyme, pceA' gene product, SDS-PAGE | Sulfurospirillum multivorans |
58000 | - |
gel filtration | Sulfurospirillum multivorans |
61000 | - |
unprocessed enzyme, expressed in Escherichia coli, SDS-PAGE | Sulfurospirillum multivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrachloroethene + reduced acceptor | Sulfurospirillum multivorans | - |
trichloroethene + chloride + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurospirillum multivorans | - |
strictly anaerobic, gram-negative bacterium | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | modified by truncation of the first 30 amino acids | Sulfurospirillum multivorans |
Purification (Comment) | Organism |
---|---|
- |
Sulfurospirillum multivorans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | chemiosmotic mechanism | Sulfurospirillum multivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrachloroethene + reduced acceptor | - |
Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
trichloroethene + reduced acceptor | - |
Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
corrinoid | corrinoid probably noncavalently bound to enzyme | Sulfurospirillum multivorans |