General Stability | Organism |
---|---|
enzyme slightly oxygen-sensitive | Sulfurospirillum multivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CCl4 | 0.1 mM: 50% inhibition | Sulfurospirillum multivorans | |
CH2Cl2 | 0.05 mM: 50% inhibition | Sulfurospirillum multivorans | |
CH3Cl | 0.0008 mM: 50% inhibition | Sulfurospirillum multivorans | |
CHCl3 | 0.025 mM: 50% inhibition | Sulfurospirillum multivorans | |
cis-1,2-dichloroethene | slight inhibition at high concentrations, 14 mM: 50% inhibition | Sulfurospirillum multivorans | |
cyanide | - |
Sulfurospirillum multivorans | |
additional information | no inhibition by 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate | Sulfurospirillum multivorans | |
Propyl iodide | 1-iodopropane; light-reversible inactivation in the presence of titanium(III)citrate as electron donor | Sulfurospirillum multivorans | |
tetrachloroethene | substrate inhibition, Ki: 18 mM | Sulfurospirillum multivorans | |
trichloroethene | substrate inhibition, Ki: 39 mM | Sulfurospirillum multivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme kinetics and kinetic parameters | Sulfurospirillum multivorans | |
0.2 | - |
tetrachloroethene | - |
Sulfurospirillum multivorans | |
0.24 | - |
trichloroethene | - |
Sulfurospirillum multivorans | |
0.28 | - |
methyl viologen | at a tetrachloroethene concentration of 1 mM | Sulfurospirillum multivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase | Sulfurospirillum multivorans | 5737 | - |
cytoplasmic membrane | studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase | Sulfurospirillum multivorans | - |
- |
cytoplasmic membrane | cytoplasmic membrane | Sulfurospirillum multivorans | - |
- |
cytoplasmic membrane | membrane-associated, periplasmic hydrogenase | Sulfurospirillum multivorans | - |
- |
additional information | cellular localization | Sulfurospirillum multivorans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | cob(II)alamin | Sulfurospirillum multivorans | |
Cobalt | cobalt content: 1.0 mol of cobalt/mol of protein | Sulfurospirillum multivorans | |
Cobalt | cobalt of the cofactor vitamin B12 appears to be subjected to a change in its redox state in the course of the halogenation | Sulfurospirillum multivorans | |
Cobalt | probably present in the 2+ oxidation state | Sulfurospirillum multivorans | |
Fe2+ | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
Fe2+ | one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
Iron-sulfur cluster | iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
Iron-sulfur cluster | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
iron-sulfur clusters | iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
iron-sulfur clusters | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
NH4+ | activation of dechlorination of perchloroethene | Sulfurospirillum multivorans | |
NH4Cl | activation of dechlorination of perchloroethene, 4 mM: 2fold activation | Sulfurospirillum multivorans | |
S | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
S | one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
1 * 57000, SDS-PAGE | Sulfurospirillum multivorans |
58000 | - |
gel filtration | Sulfurospirillum multivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrachloroethene + reduced acceptor | Sulfurospirillum multivorans | PCE, physiological electron donor is not known | trichloroethene + chloride + acceptor | - |
? | |
trichloroethene + reduced acceptor | Sulfurospirillum multivorans | physiological electron donor is not known | cis-1,2-dichloroethene + chloride + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurospirillum multivorans | - |
strictly anaerobic, gram-negative bacterium | - |
Sulfurospirillum multivorans | - |
tetrachloroethene-utilizing anaerobic bacterium | - |
Purification (Comment) | Organism |
---|---|
- |
Sulfurospirillum multivorans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | mechanism | Sulfurospirillum multivorans | |
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | thermodynamics | Sulfurospirillum multivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sulfurospirillum multivorans |
158.4 | - |
- |
Sulfurospirillum multivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity | Sulfurospirillum multivorans | ? | - |
? | |
additional information | physiological electron donor is not known | Sulfurospirillum multivorans | ? | - |
? | |
additional information | no activity with other chloroethenes, chloroethanes or chloromethanes, no activity with 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate | Sulfurospirillum multivorans | ? | - |
? | |
tetrachloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | PCE, physiological electron donor is not known | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetraiodoethene + reduced acceptor | reaction rate is considerably slower than with tetrachloroethene | Sulfurospirillum multivorans | triiodoethene + iodide + acceptor | - |
? | |
trichloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
trichloroethene + reduced acceptor | physiological electron donor is not known | Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 57000, SDS-PAGE | Sulfurospirillum multivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sulfurospirillum multivorans |
42 | - |
- |
Sulfurospirillum multivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
thermolabile above 50°C | Sulfurospirillum multivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sulfurospirillum multivorans |
8 | - |
pH-optimum about 8.0 | Sulfurospirillum multivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
corrinoid | one mol of dehalogenase contains 1.0 mol of corrinoid | Sulfurospirillum multivorans | |
corrinoid | vitamin B12 | Sulfurospirillum multivorans | |
corrinoid | dicyano-cobalamin | Sulfurospirillum multivorans | |
corrinoid | presence of a corrinoid in the cob(II)alamin form | Sulfurospirillum multivorans | |
vitamin B12 | - |
Sulfurospirillum multivorans |