Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.99.4 extracted from

  • Kuiper, G.G.; Klootwijk, W.; Visser, T.J.
    Expression of recombinant membrane-bound type I iodothyronine deiodinase in yeast (2005), J. Mol. Endocrinol., 34, 865-878.
    View publication on PubMed
Search for more literature for 1.21.99.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine, in Saccharomyces cerevisiae Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine, shows a 10fold increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate. Deletion of the endoplasmic reticulum (ER)-signal sequence and the membrane-spanning domain, amino acids 2-35, does not result in the production of a soluble type I-like enzyme. This mutant protein is inactive but is still membrane-bound Rattus norvegicus
N203Q decrease in Km-value for 3,3',5-triiodo-L-thyronine sulfate Rattus norvegicus
N94Q decrease in Km-value for 3,3',5-triiodo-L-thyronine sulfate Rattus norvegicus
N94Q/N203Q increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate Rattus norvegicus
S101A Km-value for 3,3',5-triiodo-L-thyronine sulfate is nearly identical Rattus norvegicus
S101A/S176A increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate Rattus norvegicus
Y209F/Y217F increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information mutant enzymes S101A/S176A and Y209F/Y217F show KM-values above 0.005 mM Rattus norvegicus
0.0015
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme G2A, duplicate determination give the values: 0.0015 mM and 0.0024 mM Rattus norvegicus
0.0018
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme N94Q Rattus norvegicus
0.002
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme S176A, duplicate determination give the values: 0.002 mM and 0.0024 mM Rattus norvegicus
0.002
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme S176A, duplicate determination give the values: 0.0024 mM and 0.0024 mM Rattus norvegicus
0.002
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine Rattus norvegicus
0.0022
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme S101A, duplicate determination give the values: 0.0022 mM and 3.2 mM Rattus norvegicus
0.0024
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme G2A, duplicate determination give the values: 0.0015 mM and 0.0024 mM Rattus norvegicus
0.0028
-
 3,3',5-triiodo-L-thyronine sulfate native enzyme from rat liver microsomes Rattus norvegicus
0.0029
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme N94Q/N203Q Rattus norvegicus
0.0032
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme S101A, duplicate determination give the values: 0.0022 mM and 0.0032 mM Rattus norvegicus
0.0045
-
 3,3',5-triiodo-L-thyronine sulfate mutant enzyme N203Q Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound to Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Se enzyme contains selenocysteine Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P24389
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein heterogeneity is not caused by N-linked glycosylation, but probably by a combination of O-linked glycosylation and phosphorylation Rattus norvegicus
phosphoprotein heterogeneity is not caused by N-linked glycosylation, but probably by a combination of O-linked glycosylation and phosphorylation Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5-triiodo-L-thyronine sulfate + reduced electron acceptor
-
 Rattus norvegicus diiodothyronine + iodide + electron acceptor
-
 ?

Synonyms

Synonyms Comment Organism
type I iodothyronine deiodinase
-
 Rattus norvegicus