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Literature summary for 1.21.99.4 extracted from

  • Leonard, D.M.; Stachelek, S.J.; Safran, M.; Farwell, A.P.; Kowalik, T.F.; Leonard, J.L.
    Cloning, expression, and functional characterization of the substrate binding subunit of rat type II iodothyroine 5'-deiodinase (2000), J. Biol. Chem., 275, 25194-25201.
    View publication on PubMed
Search for more literature for 1.21.99.4

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol high concentrations of cofactor increases type I activity Rattus sp.
reduced thiols e.g. dithiothreitol Rattus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli via phage lambda infection Rattus sp.
overexpression in rat astrocytes of 29 kD subunit with and without GFP-tag via adenovirus infection Rattus sp.

General Stability

General Stability Organism
very short lifetime in vivo of isoenzyme type II (D2) from brain Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information L-thyroxine inhibited competitively 3,3',5'-triiodothyronine utilization of isoenzyme type II Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
 3,3',5'-triiodothyronine recombinant isoenzyme D2 (type II) from induced brain astrocytes with cosubstrate dithiothreitol KM: 18 mM Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound, type II integral in neurolemmal membranes Rattus sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis Rattus sp.
60000
-
 several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis Rattus sp.
200000
-
-
 Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,3',5'-triiodothyronine + AH2 Rattus sp. isoenzyme type I 3,3'-diiodothyronine + iodide + A + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor type III, inner (tyrosyl) ring deiodination Rattus sp.
a
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor type II, outer ring (phenolic) deiodination Rattus sp.
a
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor type I, outer ring (phenolic) deiodination Rattus sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
brain isoenzyme D2 Rattus sp.
-
glial cell i.e. brain astrocytes in culture Rattus sp.
-
glial cell isoenzyme type II (D2) Rattus sp.
-
kidney isoenzyme type I (D1) Rattus sp.
-
liver isoenzyme typ I (D1) Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 isoenzyme D2 (type II) in cerebral cortex Rattus sp.
2
-
 isoenzyme D2 (type II) induced in cultured astrocytes Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5'-triiodothyronine + AH2 isoenzyme type I Rattus sp. 3,3'-diiodothyronine + iodide + A + H+
-
 ?

Subunits

Subunits Comment Organism
More several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis Rattus sp.

Synonyms

Synonyms Comment Organism
iodothyronine 5'-deiodinase type II, no selenoprotein, induced by bt2cAMP and hydrocortisone (ED50 4nM) Rattus sp.
iodothyronine 5'-deiodinase type I, selenoprotein, inducible by T3 (higher synthesis) and angiotensin II (positive chronotropic effect in myocard) Rattus sp.
iodothyronine 5'-deiodinase isoenzymes type I (D1) and type II (D2) Rattus sp.