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Literature summary for 1.21.99.3 extracted from

  • Martinez, L.M.; Orozco, A.; Villalobos, P.; Valverde-R, C.
    Cloning and characterization of a type 3 iodothyronine deiodinase (D3) in the liver of the chondrichtyan Chiloscyllium punctatum (2008), Gen. Comp. Endocrinol., 156, 464-469.
    View publication on PubMed
Search for more literature for 1.21.99.3

Activating Compound

Activating Compound Comment Organism Structure
DTT
-
 Chiloscyllium punctatum

Cloned(Commentary)

Cloned (Comment) Organism
expression in oocytes from Xenopus laevis Chiloscyllium punctatum

Inhibitors

Inhibitors Comment Organism Structure
6-n-propyl-2-thiouracil type 3 iodothyronine deiodinase is unaffected by PTU Chiloscyllium punctatum
Urea
-
 Chiloscyllium punctatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000001
-
 3,3',5'-triiodo-L-thyronine recombinant enzyme, in phosphate buffer Chiloscyllium punctatum
0.000002
-
 3,3',5'-triiodo-L-thyronine recombinant enzyme, with 50 mM DTT Chiloscyllium punctatum
0.0000027
-
 3,3',5'-triiodo-L-thyronine native enzyme, with 50 mM DTT Chiloscyllium punctatum
0.000005
-
 3,3',5'-triiodo-L-thyronine recombinant enzyme, in phosphate buffer, in presence of 400 mM urea Chiloscyllium punctatum
0.0000097
-
 3,3',5'-triiodo-L-thyronine recombinant enzyme, in phosphate buffer, in presence of 200 mM trimethylamine N-oxide Chiloscyllium punctatum
0.0000097
-
 3,3',5'-triiodo-L-thyronine recombinant enzyme, in phosphate buffer, in presence of 200 mM trimethylamine N-oxide and 400 mM urea Chiloscyllium punctatum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Chiloscyllium punctatum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29400
-
 calculated Chiloscyllium punctatum

Organism

Organism UniProt Comment Textmining
Chiloscyllium punctatum A9QW70 chondrichthye
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Chiloscyllium punctatum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5'-triiodo-L-thyronine + AH2 prefers T3 over T4 as substrate, bisubstrate sequential-type reaction mechanism Chiloscyllium punctatum 3,3'-diiodo-L-thyronine + iodide + A + H+
-
 ?

Synonyms

Synonyms Comment Organism
type 3 iodothyronine deiodinase sD3 Chiloscyllium punctatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Chiloscyllium punctatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Chiloscyllium punctatum

pI Value

Organism Comment pI Value Maximum pI Value
Chiloscyllium punctatum predicted
-
 7.8