Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells | Oreochromis niloticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aurothioglucose | recombinant and native isozyme type III | Oreochromis niloticus | |
iodoacetate | recombinant and native isozyme type III | Oreochromis niloticus | |
propylthiouracil | slight inhibition of recombinant and native isozyme type III | Oreochromis niloticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
3,3',5-triiodo-L-thyronine | pH 7.2, 37°C | Oreochromis niloticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | isozyme type III | Oreochromis niloticus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oreochromis niloticus | Q9I966 | isozyme type III | - |
Purification (Comment) | Organism |
---|---|
recombinant from COS-1 cells | Oreochromis niloticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isozyme type III | Oreochromis niloticus | - |
gill | gene expression | Oreochromis niloticus | - |
gut | very low activity | Oreochromis niloticus | - |
gut | isozyme type III | Oreochromis niloticus | - |
kidney | low activity | Oreochromis niloticus | - |
kidney | isozyme type III | Oreochromis niloticus | - |
liver | low activity | Oreochromis niloticus | - |
liver | isozyme type III | Oreochromis niloticus | - |
muscle | very low activity | Oreochromis niloticus | - |
muscle | isozyme type III | Oreochromis niloticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine + AH2 | best substrate | Oreochromis niloticus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodo-L-thyronine + AH2 | isozyme type III | Oreochromis niloticus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodo-L-thyronine sulfate + AH2 | isozyme type III | Oreochromis niloticus | 3,3'-diiodo-L-thyronine sulfate + iodide + A + H+ | - |
? | |
3,3',5-triiodo-L-thyronine sulfate + AH2 | about 14% of the activity with 3,3',5-triiodo-L-thyronine | Oreochromis niloticus | 3,3'-diiodo-L-thyronine sulfate + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | very low activity | Oreochromis niloticus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | isozyme type III | Oreochromis niloticus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | 5-deiodinase activity | Oreochromis niloticus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oreochromis niloticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Oreochromis niloticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | i.e. DTT | Oreochromis niloticus |