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Literature summary for 1.21.98.2 extracted from
- Enzymatic generation of the chromopyrrolic acid scaffold of rebeccamycin by the tandem action of RebO and RebD (2005), Biochemistry, 44, 15652-15663.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Lentzea aerocolonigenes |
| overproduced as a C-terminally His6-tagged protein, expression in Escherichia coli | Lentzea aerocolonigenes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | contains non-heme iron that is not part of an iron-sulfur center | Lentzea aerocolonigenes |  |
| Iron | the enzyme contains 2.93 iron centers per polypeptide | Lentzea aerocolonigenes | |
| additional information | does not contain Zn2+, Co2+, Ni2+, Mn2+, Co2+, and Mg2+ | Lentzea aerocolonigenes |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, SDS-PAGE | Lentzea aerocolonigenes |
| 110000 | - |
gel filtration | Lentzea aerocolonigenes |
| 113000 | - |
MALDI-TOF mass spectrometry | Lentzea aerocolonigenes |
| 113000 | - |
gel filtration, MALDI-TOF mass spectrometry | Lentzea aerocolonigenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | Lentzea aerocolonigenes | rebeccamycin biosynthetic pathway | 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O | - |
? | |
| 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | Lentzea aerocolonigenes ATCC 39243 | rebeccamycin biosynthetic pathway | 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentzea aerocolonigenes | Q8KHV6 | - |
- |
| Lentzea aerocolonigenes ATCC 39243 | Q8KHV6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and Superdex 200 gel filtration | Lentzea aerocolonigenes |
| purified as a C-terminally His6-tagged protein | Lentzea aerocolonigenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | - |
Lentzea aerocolonigenes | dichlorochromopyrrolate + NH3 + H2O | - |
? | |
| 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | - |
Lentzea aerocolonigenes ATCC 39243 | dichlorochromopyrrolate + NH3 + H2O | - |
? | |
| 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | - |
Lentzea aerocolonigenes | 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O | - |
? | |
| 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | rebeccamycin biosynthetic pathway | Lentzea aerocolonigenes | 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O | - |
? | |
| 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | - |
Lentzea aerocolonigenes ATCC 39243 | 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O | - |
? | |
| 4 2-imino-3-(7-chloroindol-3-yl)propanoate + O2 | rebeccamycin biosynthetic pathway | Lentzea aerocolonigenes ATCC 39243 | 2 dichlorochromopyrrolate + 2 NH3 + 2 H2O | - |
? | |
| additional information | RebD acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and water | Lentzea aerocolonigenes | ? | - |
? | |
| additional information | the enzyme has both catalase and chromopyrrolic acid synthase activity. The enzyme acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and (presumably) water. The enzyme on its own is incompetent with L-tryptophan | Lentzea aerocolonigenes | ? | - |
? | |
| additional information | RebD acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and water | Lentzea aerocolonigenes ATCC 39243 | ? | - |
? | |
| additional information | the enzyme has both catalase and chromopyrrolic acid synthase activity. The enzyme acts as an efficient catalase, effecting the disproportionation of hydrogen peroxide to give oxygen and (presumably) water. The enzyme on its own is incompetent with L-tryptophan | Lentzea aerocolonigenes ATCC 39243 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Lentzea aerocolonigenes |
| dimer | 2 * 56000, SDS-PAGE | Lentzea aerocolonigenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RebD | - |
Lentzea aerocolonigenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Lentzea aerocolonigenes | |
| heme | contains one molecule of heme b per monomer | Lentzea aerocolonigenes | |
| heme | the enzyme contains 1.8 heme centers per polypeptide | Lentzea aerocolonigenes | |
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Release 2023.1 (January 2023)
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