Protein Variants | Comment | Organism |
---|---|---|
C13S | the mutant enzyme shows no affinity for the glutathione-Sepharose affinity matrix. The mutant enzyme is not inhibited by 2,3,6-trichlorohydroquinone | Sphingobium chlorophenolicum |
C156S | experiments are carried out using C156S TCHQ dehalogenase, which has only one Cys residue at position 13 in the active site, in order to avoid complications in the synthesis of ESSG arising from reactions with Cys156, which is located on the surface at a distance from the active site. The substitution of Ser for Cys at position 156 does not appear to affect any properties of the enzyme | Sphingobium chlorophenolicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,5,6-tetrachlorohydroquinone | inhibits the wild-typ enzyme and not the mutant enzyme | Sphingobium chlorophenolicum | |
2,3,6-trichlorohydroquinone | noncompetitive inhibitor of the thiol-disulfide exchange reaction, in addition, dissociation of the GSSG product is inhibited by 2,3,6-trichlorohydroquinone | Sphingobium chlorophenolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2,3,5,6-tetrachlorohydroquinone | pH 7.0, 20°C, mutant enzyme C13S | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | Sphingobium chlorophenolicum | the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | Sphingobium chlorophenolicum | the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | Q03520 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative | Sphingobium chlorophenolicum | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | the first glutathione is involved in formation of a glutathione conjugate, while the second is involved in the final step of the reaction, a thiol-disulfide exchange reaction that regenerates the free enzyme and forms GSSG. The thiol-disulfide exchange reaction is the rate-limiting step in the reductive dehalogenation reaction under physiological conditions | Sphingobium chlorophenolicum | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative | Sphingobium chlorophenolicum | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | the first glutathione is involved in formation of a glutathione conjugate, while the second is involved in the final step of the reaction, a thiol-disulfide exchange reaction that regenerates the free enzyme and forms GSSG. The thiol-disulfide exchange reaction is the rate-limiting step in the reductive dehalogenation reaction under physiological conditions | Sphingobium chlorophenolicum | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Sphingobium chlorophenolicum |
Synonyms | Comment | Organism |
---|---|---|
TCHQ dehalogenase | - |
Sphingobium chlorophenolicum |
tetrachlorohydroquinone dehalogenase | - |
Sphingobium chlorophenolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
2,3,5,6-tetrachlorohydroquinone | pH 7.0, 20°C, mutant enzyme C13SS | Sphingobium chlorophenolicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
2,3,5,6-tetrachlorohydroquinone | pH 7.0, 20°C, mutant enzyme C156S | Sphingobium chlorophenolicum |