Activating Compound | Comment | Organism | Structure |
---|---|---|---|
O2 | required | Aspergillus nidulans | |
O2 | required | Acremonium chrysogenum | |
O2 | required | Streptomyces jumonjinensis |
Crystallization (Comment) | Organism |
---|---|
structure analysis | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
D214H | site-directed mutagenesis, complete inactive enzyme | Streptomyces jumonjinensis |
additional information | exchange of Asp214 and His268, and exchange of Asp214 and His 212 by site-directed mutagenesis leads to completely inactive enzymes | Streptomyces jumonjinensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Aspergillus nidulans | |
Fe2+ | required | Acremonium chrysogenum | |
Fe2+ | required | Streptomyces jumonjinensis | |
Fe2+ | iron-binding motif | Streptomyces jumonjinensis | |
Fe2+ | structure of the Fe2+ active site and endogenous ligands | Aspergillus nidulans | |
Fe2+ | structure of the Fe2+ active site and endogenous ligands | Acremonium chrysogenum | |
Fe2+ | structure of the Fe2+ active site and endogenous ligands | Streptomyces jumonjinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Aspergillus nidulans | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Acremonium chrysogenum | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Streptomyces jumonjinensis | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Aspergillus nidulans | key enzyme of the biosynthetic pathway | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Acremonium chrysogenum | key enzyme of the biosynthetic pathway | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Streptomyces jumonjinensis | key enzyme of the biosynthetic pathway | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
- |
- |
Aspergillus nidulans | - |
- |
- |
Aspergillus nidulans | P05326 | - |
- |
Streptomyces jumonjinensis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O | structure and mechanism | Aspergillus nidulans | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O | structure and mechanism | Acremonium chrysogenum | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O | structure and mechanism | Streptomyces jumonjinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Aspergillus nidulans | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Acremonium chrysogenum | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Streptomyces jumonjinensis | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Aspergillus nidulans | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Acremonium chrysogenum | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Streptomyces jumonjinensis | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | key enzyme of the biosynthetic pathway | Aspergillus nidulans | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | key enzyme of the biosynthetic pathway | Acremonium chrysogenum | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | key enzyme of the biosynthetic pathway | Streptomyces jumonjinensis | isopenicillin N + 2 H2O | product has antibiotic activity | ? |
Synonyms | Comment | Organism |
---|---|---|
IPNS | - |
Aspergillus nidulans |
IPNS | - |
Acremonium chrysogenum |
IPNS | - |
Streptomyces jumonjinensis |