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Literature summary for 1.21.3.1 extracted from
- Genetic and biochemical analysis of the cysteinyl residues of isopenicillin N synthase from Streptomyces clavuligerus (1996), Can. J. Microbiol., 42, 870-875.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | - |
Streptomyces clavuligerus |  |
| dithiothreitol | - |
Streptomyces clavuligerus | |
| O2 | required | Streptomyces clavuligerus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutants in Escherichia coli | Streptomyces clavuligerus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C104S | single-strand-site-directed mutagenesis, loss of more than 96% activity | Streptomyces clavuligerus |
| C142S | single-strand-site-directed mutagenesis, loss of 24% activity | Streptomyces clavuligerus |
| C251S | single-strand-site-directed mutagenesis, loss of 47.7% activity | Streptomyces clavuligerus |
| C37S | single-strand-site-directed mutagenesis, loss of 18.3% activity | Streptomyces clavuligerus |
| C37S/C142S/C251S | triple mutant, conformationally different from wild-type, prepared by recombining fragments of IPNS-encoding gene pcbC from each of the single mutants, loss of more than 90% activity | Streptomyces clavuligerus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | wild-type and mutants, binds to Cys104 in the active site | Streptomyces clavuligerus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Streptomyces clavuligerus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Streptomyces clavuligerus | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, wild-type and mutants recombinant from Escherichia coli | Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000000125 | - |
mutant C104S, recombinant, partially purified | Streptomyces clavuligerus |
| 0.000000177 | - |
mutant C251S, recombinant, partially purified | Streptomyces clavuligerus |
| 0.000000257 | - |
mutant C142S, recombinant, partially purified | Streptomyces clavuligerus |
| 0.000000276 | - |
mutant C37S, recombinant, partially purified | Streptomyces clavuligerus |
| 0.000000338 | - |
wild-type, recombinant, partially purified | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Streptomyces clavuligerus | isopenicillin N + 2 H2O | - |
? | |
| N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Streptomyces clavuligerus | isopenicillin N + 2 H2O | product has antibiotic activity | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IPNS | - |
Streptomyces clavuligerus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
assay at | Streptomyces clavuligerus |
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