Literature summary for 1.21.1.1 extracted from

Thomas, S.R.; McTamney, P.M.; Adler, J.M.; Laronde-Leblanc, N.; Rokita, S.E.
Crystal structure of iodotyrosine deiodinase, a novel flavoprotein responsible for iodide salvage in thyroid glands (2009), J. Biol. Chem., 284, 19659-19667.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of a derivative lacking codons for amino acids 233 to gain a soluble protein	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of soluble enzyme lacking codons for amino acids 2-33 and two co-crystals containing substrates, mono- and diiodotyrosine, alternatively, at resolutions of 2.0 A, 2.45 A, and 2.6 A, respectively. Substrate coordination induces formation of an additional helix and coil that act as an active site lid to shield the resulting substrateflavin complex from solvent. This complex is stabilized by aromatic stacking and extensive hydrogen bonding between the substrate and flavin. The carbon-iodine bond of the substrate is positioned directly over the C-4a/N-5 region of the flavin to promote electron transfer	Mus musculus

Crystallization (Comment)

Organism

structures of soluble enzyme lacking codons for amino acids 2-33 and two co-crystals containing substrates, mono- and diiodotyrosine, alternatively, at resolutions of 2.0 A, 2.45 A, and 2.6 A, respectively. Substrate coordination induces formation of an additional helix and coil that act as an active site lid to shield the resulting substrateflavin complex from solvent. This complex is stabilized by aromatic stacking and extensive hydrogen bonding between the substrate and flavin. The carbon-iodine bond of the substrate is positioned directly over the C-4a/N-5 region of the flavin to promote electron transfer

Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q9DCX8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3,5-diiodo-L-tyrosine + NADPH + H+	-	Mus musculus	3-iodo-L-tyrosine + NADP+ + I-	-	?
3-iodo-L-tyrosine + NADPH + H+	-	Mus musculus	L-tyrosine + NADP+ + I-	-	?

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Release 2023.1 (January 2023)