Literature summary for 1.20.99.1 extracted from

Bhattacharjee, H.; Sheng, J.; Ajees, A.; Mukhopadhyay, R.; Rosen, B.
Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B and Cdc25C phosphatases (2010), Biochemistry, 49, 802-809.

Search for more literature for 1.20.99.1

Cloned(Commentary)

Cloned (Comment)	Organism
catalytic domain is expressed in Escherichia coli as a His-tagged fusion protein	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
C120A	inactive mutant	Homo sapiens
additional information	catalytic domain of Cdc25B is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals. Alteration of either the cysteine and arginine residues of the Cys-X5-Arg motif leads to the loss of both reductase and phosphatase activities	Homo sapiens
R126A	inactive mutant	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.9	-	arsenate	pH 8.5, 30°C, 5 mM glutathione	Homo sapiens
1.7	-	arsenate	pH 8.5, 30°C, 2 mM glutathione	Homo sapiens
2.5	-	arsenate	pH 8.5, 30°C, 5 mM glutathione	Homo sapiens
5	-	arsenate	pH 8.5, 30°C, 2 mM glutathione	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + reduced acceptor	in the presence of glutathione, human glutaredoxin, and human glutathione reductase, Cdc25B is found to catalyze the reduction of As(V) to As(III)	Homo sapiens	arsenite + acceptor	-	?

Synonyms

Synonyms	Comment	Organism
Cdc25B	isoform of human Cdc25 phosphatase is homologous to eukaryotic arsenate reductase and possesses arsenate reductase activity	Homo sapiens
Cdc25C	isoform of human Cdc25 phosphatase is homologous to eukaryotic arsenate reductase and possesses arsenate reductase activity	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at and optimal temperature	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.012	-	arsenate	pH 8.5, 30°C, 2 mM glutathione	Homo sapiens
0.017	-	arsenate	pH 8.5, 30°C, 2 mM glutathione	Homo sapiens
0.025	-	arsenate	pH 8.5, 30°C, 5 mM glutathione	Homo sapiens
0.03	-	arsenate	pH 8.5, 30°C, 5 mM glutathione	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	catalytic domain of Cdc25C is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals	additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0034	-	arsenate	pH 8.5, 30°C, 2 mM glutathione	Homo sapiens
0.007	-	arsenate	pH 8.5, 30°C, 2 mM glutathione	Homo sapiens
0.012	-	arsenate	pH 8.5, 30°C, 5 mM glutathione	Homo sapiens
0.027	-	arsenate	pH 8.5, 30°C, 5 mM glutathione	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)