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Literature summary for 1.20.4.4 extracted from

  • Villadangos, A.F.; Van Belle, K.; Wahni, K.; Dufe, V.T.; Freitas, S.; Nur, H.; De Galan, S.; Gil, J.A.; Collet, J.F.; Mateos, L.M.; Messens, J.
    Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms (2011), Mol. Microbiol., 82, 998-1014.
    View publication on PubMed
Search for more literature for 1.20.4.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment) Organism
seleno-methionine derivative, to 2.2 A resolution Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information in mutant lacking N-terminal 78 amino acids, the kinetic parameters are greatly reduced Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 arsenate wild-type, pH 8.0, 37°C Corynebacterium glutamicum
0.11
-
 arsenate N-terminally truncated mutant, pH 8.0, 37°C Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25360
-
 2 * 25360, SDS-PAGE Corynebacterium glutamicum
60000
-
 gel filtration Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum P0DKS5 enzyme additionally shows low phosphates activity
-
Corynebacterium glutamicum DSM 20300 P0DKS5 enzyme additionally shows low phosphates activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + thioredoxin
-
 Corynebacterium glutamicum arsenite + thioredoxin disulfide + H2O
-
 ?
arsenate + thioredoxin
-
 Corynebacterium glutamicum DSM 20300 arsenite + thioredoxin disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 25360, SDS-PAGE Corynebacterium glutamicum
More isoform ArsC1' is unique in bearing an N-terminal three-helical bundle that interacts with the active site of the other chain in the dimeric interface Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
ArsC1'
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 arsenate wild-type, pH 8.0, 37°C Corynebacterium glutamicum
0.5
-
 arsenate N-terminally truncated mutant, pH 8.0, 37°C Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: mycoredoxin Corynebacterium glutamicum
thioredoxin
-
 Corynebacterium glutamicum

Expression

Organism Comment Expression
Corynebacterium glutamicum isooform ArsC1' is constitutively expressed at low levels using its own promoter site. It reduces arsenate to arsenite that can then induce the expression of isoforms ArsC1 and ArsC2 additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.045
-
 arsenate N-terminally truncated mutant, pH 8.0, 37°C Corynebacterium glutamicum
2.9
-
 arsenate wild-type, pH 8.0, 37°C Corynebacterium glutamicum