Literature summary for 1.20.4.4 extracted from

Roos, G.; Buts, L.; Van Belle, K.; Brosens, E.; Geerlings, P.; Loris, R.; Wyns, L.; Messens, J.
Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family (2006), J. Mol. Biol., 360, 826-838.

Search for more literature for 1.20.4.4

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	mechanism by which potassium and tetrahedral oxyanions affect catalysis within the Trx-coupled family of arsenate reductases	Staphylococcus aureus
additional information	mechanism by which potassium and tetrahedral oxyanions affect catalysis within the Trx-coupled family of arsenate reductases	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Staphylococcus aureus
expressed in Escherichia coli	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
x-ray structures of the mutants	Staphylococcus aureus
x-ray structures of the mutants	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
C10S/C15A	change of the P-loop geometry	Staphylococcus aureus
H62Q	change of the P-loop geometry	Staphylococcus aureus
H62Q/N33K	site directed mutagenesis	Staphylococcus aureus
H62Q/N33K/E30D/G31E	site directed mutagenesis	Staphylococcus aureus
K33D	site directed mutagenesis	Bacillus subtilis
K33N	site directed mutagenesis	Bacillus subtilis
additional information	commonly occurring mutation of a histidine (H62), located about 6 A from the potassium-binding site in Sa_ArsC, to a glutamine uncouples the kinetic dependency on potassium. Mutations within the Trx-coupled family of arsenate reductases lead to subtly different ion-dependent kinetic features	Staphylococcus aureus
additional information	commonly occurring mutation of a histidine (H62), located about 6 A from the potassium-binding site in Sa_ArsC, to a glutamine uncouples the kinetic dependency on potassium. Mutations within the Trx-coupled family of arsenate reductases lead to subtly different ion-dependent kinetic features	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009	-	arsenate	wild-type Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.022	-	arsenate	wild-type Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.047	-	arsenate	wild-type Bacillus subtilis, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
0.054	-	arsenate	wild-type Bacillus subtilis, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
0.058	-	arsenate	wild-type Bacillus subtilis, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
0.061	-	arsenate	wild-type Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.064	-	arsenate	wild-type Bacillus subtilis, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
0.08	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.081	-	arsenate	wild-type Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.087	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.123	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.131	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P45947	-	-
Staphylococcus aureus	P0A006	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus
-	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + thioredoxin	-	Staphylococcus aureus	arsenite + thioredoxin disulfide + H2O	-	?
arsenate + thioredoxin	-	Bacillus subtilis	arsenite + thioredoxin disulfide + H2O	-	?

Synonyms

Synonyms	Comment	Organism
Bs_ArsC	-	Bacillus subtilis
Sa_ArsC	-	Staphylococcus aureus
thioredoxin-coupled arsenate reductase	detoxification enzyme	Staphylococcus aureus
thioredoxin-coupled arsenate reductase	detoxification enzyme	Bacillus subtilis
Trx-coupled arsenate reductase	-	Staphylococcus aureus
Trx-coupled arsenate reductase	-	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.58	-	arsenate	wild-type Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.7	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.75	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
0.9	-	arsenate	wild-type Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
1.3	-	arsenate	wild-type Bacillus subtilis, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
1.6	-	arsenate	wild-type Bacillus subtilis, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
1.6	-	arsenate	wild-type Bacillus subtilis, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
1.98	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
2	-	arsenate	wild-type Bacillus subtilis, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
2.9	-	arsenate	wild-type Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
3.03	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
3.65	-	arsenate	wild-type Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.1	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
8.1	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
22.4	-	arsenate	wild-type Bacillus subtilis, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
23.1	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
24.8	-	arsenate	H62Q mutant Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
26.4	-	arsenate	wild-type Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
29.6	-	arsenate	wild-type Bacillus subtilis, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
31.3	-	arsenate	wild-type Bacillus subtilis, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
34	-	arsenate	wild-type Bacillus subtilis, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Bacillus subtilis
45.1	-	arsenate	wild-type Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
47.5	-	arsenate	wild-type Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus
100	-	arsenate	wild-type Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters	Staphylococcus aureus

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Release 2023.1 (January 2023)