Literature summary for 1.20.4.1 extracted from

  • Duan, G.L.; Zhu, Y.G.; Tong, Y.P.; Cai, C.; Kneer, R.
    Characterization of arsenate reductase in the extract of roots and fronds of Chinese brake fern, an arsenic hyperaccumulator (2005), Plant Physiol., 138, 461-469.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is arsenate-inducible Pteris vittata

Protein Variants

Protein Variants Comment Organism
additional information a disruption mutant is inactive Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
arsenite noncompetitive Pteris vittata
phosphate competitive Pteris vittata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
arsenate pH 6.5, 30°C Pteris vittata
0.57
-
reduced glutathione pH 6.5, 30°C Pteris vittata
2.33
-
arsenate pH 6.5, 30°C Pteris vittata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arsenate + reduced acceptor Arabidopsis thaliana
-
arsenite + acceptor
-
?
arsenate + reduced acceptor Oryza sativa
-
arsenite + acceptor
-
?
arsenate + reduced glutathione Pteris vittata enzyme plays an important role in detoxification of arsenate arsenite + glutathione
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Oryza sativa
-
-
-
Pteris vittata
-
Chinese brake fern, an arsenic hyperaccumulator
-
Pteris vittata
-
5fold increase in enzyme activity after 9 days treatment with 2 mM arsenate
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Arabidopsis thaliana
-
root
-
Oryza sativa
-
root
-
Pteris vittata
-
additional information no activity in shoot, arsenite is the main storage form in the fern fronds Pteris vittata
-
additional information no enzymic activity in shoots Pteris vittata
-
shoot
-
Arabidopsis thaliana
-
shoot
-
Oryza sativa
-
frond
-
Pteris vittata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
in vivo activity after feeding experiments Pteris vittata
2.45
-
root Oryza sativa
24
-
isolated roots in arsenate containiing culture medium, induced for 9 h by arsenate Pteris vittata
3.84
-
shoot Oryza sativa
4.8
-
isolated roots in arsenate containiing culture medium, basic level Pteris vittata
2
-
shoot Arabidopsis thaliana
3.55
-
root Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: phosphate, nitrate Pteris vittata ?
-
?
arsenate + reduced acceptor
-
Arabidopsis thaliana arsenite + acceptor
-
?
arsenate + reduced acceptor
-
Oryza sativa arsenite + acceptor
-
?
arsenate + reduced glutathione
-
Arabidopsis thaliana arsenite + glutathione
-
?
arsenate + reduced glutathione
-
Oryza sativa arsenite + glutathione
-
?
arsenate + reduced glutathione
-
Pteris vittata arsenite + glutathione
-
?
arsenate + reduced glutathione enzyme plays an important role in detoxification of arsenate Pteris vittata arsenite + glutathione
-
?

Synonyms

Synonyms Comment Organism
arsenate reductase
-
Arabidopsis thaliana
arsenate reductase
-
Oryza sativa
arsenate reductase
-
Pteris vittata
AR
-
Arabidopsis thaliana
AR
-
Oryza sativa
AR
-
Pteris vittata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana
30
-
assay at Oryza sativa
20 50 broad optimum Pteris vittata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
Pteris vittata
20 50 broad Pteris vittata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Pteris vittata
6.5
-
broad optimum Pteris vittata
6.5
-
assay at Arabidopsis thaliana
6.5
-
assay at Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7
-
Pteris vittata
5.5 7.5 90% of maximal activity at pH 5.5, 60% at pH 7.5 Pteris vittata
7.5
-
60% of maximum activity Pteris vittata
5.5
-
90% of maximum activity Pteris vittata

Cofactor

Cofactor Comment Organism Structure
glutathione
-
Arabidopsis thaliana
glutathione
-
Oryza sativa
glutathione
-
Pteris vittata