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Literature summary for 1.20.4.1 extracted from
- Characterization of arsenate reductase in the extract of roots and fronds of Chinese brake fern, an arsenic hyperaccumulator (2005), Plant Physiol., 138, 461-469.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is arsenate-inducible | Pteris vittata |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a disruption mutant is inactive | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | noncompetitive | Pteris vittata |  |
| phosphate | competitive | Pteris vittata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
reduced glutathione | pH 6.5, 30°C | Pteris vittata | |
| 2.3 | - |
arsenate | pH 6.5, 30°C | Pteris vittata | |
| 2.33 | - |
arsenate | pH 6.5, 30°C | Pteris vittata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + reduced acceptor | Arabidopsis thaliana | - |
arsenite + acceptor | - |
? | |
| arsenate + reduced acceptor | Oryza sativa | - |
arsenite + acceptor | - |
? | |
| arsenate + reduced glutathione | Pteris vittata | enzyme plays an important role in detoxification of arsenate | arsenite + glutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Oryza sativa | - |
- |
- |
| Pteris vittata | - |
Chinese brake fern, an arsenic hyperaccumulator | - |
| Pteris vittata | - |
5fold increase in enzyme activity after 9 days treatment with 2 mM arsenate | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H2O | reaction mechanism | Pteris vittata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| frond | - |
Pteris vittata | - |
| additional information | no activity in shoot, arsenite is the main storage form in the fern fronds | Pteris vittata | - |
| additional information | no enzymic activity in shoots | Pteris vittata | - |
| root | - |
Arabidopsis thaliana | - |
| root | - |
Oryza sativa | - |
| root | - |
Pteris vittata | - |
| shoot | - |
Arabidopsis thaliana | - |
| shoot | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
in vivo activity after feeding experiments | Pteris vittata |
| 2 | - |
shoot | Arabidopsis thaliana |
| 2.45 | - |
root | Oryza sativa |
| 3.55 | - |
root | Arabidopsis thaliana |
| 3.84 | - |
shoot | Oryza sativa |
| 4.8 | - |
isolated roots in arsenate containiing culture medium, basic level | Pteris vittata |
| 24 | - |
isolated roots in arsenate containiing culture medium, induced for 9 h by arsenate | Pteris vittata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + reduced acceptor | - |
Arabidopsis thaliana | arsenite + acceptor | - |
? | |
| arsenate + reduced acceptor | - |
Oryza sativa | arsenite + acceptor | - |
? | |
| arsenate + reduced glutathione | - |
Arabidopsis thaliana | arsenite + glutathione | - |
? | |
| arsenate + reduced glutathione | - |
Oryza sativa | arsenite + glutathione | - |
? | |
| arsenate + reduced glutathione | - |
Pteris vittata | arsenite + glutathione | - |
? | |
| arsenate + reduced glutathione | enzyme plays an important role in detoxification of arsenate | Pteris vittata | arsenite + glutathione | - |
? | |
| additional information | no substrate: phosphate, nitrate | Pteris vittata | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AR | - |
Arabidopsis thaliana |
| AR | - |
Oryza sativa |
| AR | - |
Pteris vittata |
| arsenate reductase | - |
Arabidopsis thaliana |
| arsenate reductase | - |
Oryza sativa |
| arsenate reductase | - |
Pteris vittata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | broad optimum | Pteris vittata |
| 30 | - |
assay at | Arabidopsis thaliana |
| 30 | - |
assay at | Oryza sativa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Pteris vittata |
| 20 | 50 | broad | Pteris vittata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pteris vittata |
| 6.5 | - |
broad optimum | Pteris vittata |
| 6.5 | - |
assay at | Arabidopsis thaliana |
| 6.5 | - |
assay at | Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Pteris vittata |
| 5.5 | 7.5 | 90% of maximal activity at pH 5.5, 60% at pH 7.5 | Pteris vittata |
| 5.5 | - |
90% of maximum activity | Pteris vittata |
| 7.5 | - |
60% of maximum activity | Pteris vittata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | - |
Arabidopsis thaliana | |
| glutathione | - |
Oryza sativa | |
| glutathione | - |
Pteris vittata | |
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