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Literature summary for 1.20.4.1 extracted from

  • Mukhopadhyay, R.; Shi, J.; Rosen, B.P.
    Purification and characterization of Acr2p, the Saccharomyces cerevisiae arsenate reductase (2000), J. Biol. Chem., 275, 21149-21157.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GSH required Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the bacterial expression vector and expressed in Escherichia coli as a C-terminal histidine-tagged protein Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
arsenite
-
Saccharomyces cerevisiae
phosphate poor Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
glutaredoxin glutaredoxin 1p from Saccharomyces cerevisiae or glutaredoxin 1 from E. coli Saccharomyces cerevisiae
0.0001
-
glutaredoxin glutaredoxin 3 from E. coli Saccharomyces cerevisiae
0.000002
-
glutaredoxin glutaredoxin 2 from E. coli Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration Saccharomyces cerevisiae
16822
-
2 * 16822, calculation from nucleotide sequence Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + reduced glutaredoxin thioredoxin is unable to support arsenate reduction. The N-terminal Cys residue is essential for arsenate reductase activity. During the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced vby glutaredoxin to regenerate the active Acr2p reductase Saccharomyces cerevisiae arsenite + oxidized glutaredoxin
-
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Subunits

Subunits Comment Organism
dimer 2 * 16822, calculation from nucleotide sequence Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6.5
-
Saccharomyces cerevisiae