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Literature summary for 1.20.1.1 extracted from

  • Beyer, N.; Kulig, J.K.; Bartsch, A.; Hayes, M.A.; Janssen, D.B.; Fraaije, M.W.
    P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations (2017), Appl. Microbiol. Biotechnol., 101, 2319-2331 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.20.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.201
-
 phosphonate at pH 7.5 and 30°C Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphonate + NAD+ + H2O Pseudomonas stutzeri
-
 phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O Pseudomonas stutzeri WM88
-
 phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri WM88
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri WM88 phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
phosphite dehydrogenase
-
 Pseudomonas stutzeri
PTDH
-
 Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.87
-
 phosphonate at pH 7.5 and 30°C Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.3
-
 phosphonate at pH and °C Pseudomonas stutzeri