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Literature summary for 1.20.1.1 extracted from

  • Hung, J.E.; Fogle, E.J.; Christman, H.D.; Johannes, T.W.; Zhao, H.; Metcalf, W.W.; van der Donk, W.A.
    Investigation of the role of Arg301 identified in the X-ray structure of phosphite dehydrogenase (2012), Biochemistry, 51, 4254-4262.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.20.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methylorubrum extorquens
-
 Nostoc punctiforme
-
 Cupriavidus metallidurans
-
 Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
additional information mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ Methylorubrum extorquens
additional information mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ Nostoc punctiforme
additional information mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ Cupriavidus metallidurans
additional information mutant 17x-PTDH is based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+ Pseudomonas stutzeri
R301A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type Methylorubrum extorquens
R301A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type Nostoc punctiforme
R301A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type Cupriavidus metallidurans
R301A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301A leads to sharp decrease in activity, while kcat value is similar to wild-type Pseudomonas stutzeri
R301K mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type Methylorubrum extorquens
R301K mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type Nostoc punctiforme
R301K mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type Cupriavidus metallidurans
R301K mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation R301K leads to sharp decrease in activity, while kcat value is similar to wild-type Pseudomonas stutzeri
S295A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type Methylorubrum extorquens
S295A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type Nostoc punctiforme
S295A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type Cupriavidus metallidurans
S295A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation S295A leads to sharp decrease in activity, while kcat value is similar to wild-type Pseudomonas stutzeri
W134A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type Methylorubrum extorquens
W134A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type Nostoc punctiforme
W134A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type Cupriavidus metallidurans
W134A mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134A leads to sharp decrease in activity, while kcat value is similar to wild-type Pseudomonas stutzeri
W134F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type Methylorubrum extorquens
W134F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type Nostoc punctiforme
W134F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type Cupriavidus metallidurans
W134F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation W134F leads to sharp decrease in activity, while kcat value is similar to wild-type Pseudomonas stutzeri
Y139F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type Methylorubrum extorquens
Y139F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type Nostoc punctiforme
Y139F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type Cupriavidus metallidurans
Y139F mutant based on a thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity, plus mutation E175A that allows this mutant to use both NAD+ and NADP+. Mutation Y139F leads to sharp decrease in activity, while kcat value is similar to wild-type Pseudomonas stutzeri

Inhibitors

Inhibitors Comment Organism Structure
NAD+
-
 Cupriavidus metallidurans
NAD+
-
 Methylorubrum extorquens
NAD+
-
 Nostoc punctiforme
NAD+
-
 Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 NAD+ pH 7.3, 25°C Cupriavidus metallidurans
0.0194
-
 NAD+ pH 7.3, 25°C Nostoc punctiforme
0.022
-
 NAD+ mutant 17X-PTDH, pH 7.3, 25°C Pseudomonas stutzeri
0.0254
-
 phosphonate pH 7.3, 25°C Nostoc punctiforme
0.028
-
 phosphonate mutant 17X-PTDH, pH 7.3, 25°C Pseudomonas stutzeri
0.036
-
 NAD+ mutant Y139F, pH 7.3, 25°C Pseudomonas stutzeri
0.039
-
 phosphonate mutant Y139F, pH 7.3, 25°C Pseudomonas stutzeri
0.0405
-
 NAD+ pH 7.3, 25°C Methylorubrum extorquens
0.0522
-
 NAD+ wild-type, pH 7.3, 25°C Pseudomonas stutzeri
0.0549
-
 phosphonate pH 7.3, 25°C Cupriavidus metallidurans
0.074
-
 NAD+ mutant R301K, pH 7.3, 25°C Pseudomonas stutzeri
0.0811
-
 phosphonate wild-type, pH 7.3, 25°C Pseudomonas stutzeri
0.102
-
 NAD+ mutant W134F, pH 7.3, 25°C Pseudomonas stutzeri
0.103
-
 phosphonate mutant S295A, pH 7.3, 25°C Pseudomonas stutzeri
0.187
-
 NAD+ mutant W134A, pH 7.3, 25°C Pseudomonas stutzeri
0.216
-
 NAD+ mutant R301A, pH 7.3, 25°C Pseudomonas stutzeri
0.237
-
 phosphonate pH 7.3, 25°C Methylorubrum extorquens
0.238
-
 phosphonate mutant W134F, pH 7.3, 25°C Pseudomonas stutzeri
0.25
-
 NAD+ mutant S295A, pH 7.3, 25°C Pseudomonas stutzeri
1.11
-
 phosphonate mutant R301K, pH 7.3, 25°C Pseudomonas stutzeri
1.91
-
 phosphonate mutant W134A, pH 7.3, 25°C Pseudomonas stutzeri
19.4
-
 phosphonate mutant R301A, pH 7.3, 25°C Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Cupriavidus metallidurans
-
-
-
Cupriavidus metallidurans CH34
-
-
-
Methylorubrum extorquens
-
-
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-
Nostoc punctiforme
-
-
-
Pseudomonas stutzeri O69054
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphonate + NAD+ + H2O
-
 Methylorubrum extorquens phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Nostoc punctiforme phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Cupriavidus metallidurans phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Cupriavidus metallidurans CH34 phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
PTDH
-
 Methylorubrum extorquens
PTDH
-
 Nostoc punctiforme
PTDH
-
 Cupriavidus metallidurans
PTDH
-
 Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.041
-
 phosphonate mutant R301A, pH 7.3, 25°C Pseudomonas stutzeri
1.73
-
 phosphonate mutant W134A, pH 7.3, 25°C Pseudomonas stutzeri
2.53
-
 phosphonate mutant S295A, pH 7.3, 25°C Pseudomonas stutzeri
2.96
-
 phosphonate mutant W134F, pH 7.3, 25°C Pseudomonas stutzeri
3.27
-
 phosphonate mutant 17X-PTDH, pH 7.3, 25°C Pseudomonas stutzeri
3.36
-
 phosphonate pH 7.3, 25°C Nostoc punctiforme
3.68
-
 phosphonate mutant Y139F, pH 7.3, 25°C Pseudomonas stutzeri
4.5
-
 phosphonate mutant R301K, pH 7.3, 25°C Pseudomonas stutzeri
5.1
-
 phosphonate pH 7.3, 25°C Methylorubrum extorquens
6.57
-
 phosphonate wild-type, pH 7.3, 25°C Pseudomonas stutzeri
6.83
-
 phosphonate pH 7.3, 25°C Cupriavidus metallidurans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.48
-
 NAD+ mutant R301A, pH 7.3, 25°C Pseudomonas stutzeri
0.97
-
 NAD+ mutant R301K, pH 7.3, 25°C Pseudomonas stutzeri
1.03
-
 NAD+ mutant 17X-PTDH, pH 7.3, 25°C Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00211
-
 phosphonate mutant R301A, pH 7.3, 25°C Pseudomonas stutzeri
0.901
-
 phosphonate mutant W134A, pH 7.3, 25°C Pseudomonas stutzeri
4.05
-
 phosphonate mutant R301K, pH 7.3, 25°C Pseudomonas stutzeri
12.4
-
 phosphonate mutant W134F, pH 7.3, 25°C Pseudomonas stutzeri
24.6
-
 phosphonate mutant S295A, pH 7.3, 25°C Pseudomonas stutzeri
94.4
-
 phosphonate mutant Y139F, pH 7.3, 25°C Pseudomonas stutzeri
117
-
 phosphonate mutant 17X-PTDH, pH 7.3, 25°C Pseudomonas stutzeri