Literature summary for 1.2.99.8 extracted from

Wakagi, T.; Nishimasu, H.; Miyake, M.; Fushinobu, S.
Archaeal Mo-containing glyceraldehyde oxidoreductase isozymes exhibit diverse substrate specificities through unique subunit assemblies (2016), PLoS ONE, 11, e0147333.

Search for more literature for 1.2.99.8

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.2 A resolution. The asymmetric unit contains one hetero-trimer, one Mo-PCD (large subunit), one FAD (medium subunit), two [2Fe-2S] clusters (designated as I and II, small subunit), and solvent molecules	Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0035	-	D-Glyceraldehyde-3-phosphate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.011	-	D-glyceraldehyde	cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.012	-	D-Glyceraldehyde-3-phosphate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.018	-	D-glyceraldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.03	-	D-glyceraldehyde	cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.037	-	Indoleacetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.085	-	D-Glyceraldehyde-3-phosphate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.091	-	D-glyceraldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.092	-	Indoleacetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.113	-	Indoleacetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.138	-	D-glyceraldehyde	cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.24	-	acetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.63	-	D-glyceraldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
1.33	-	acetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
1.86	-	Indolepyruvate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	0.3 nmol/mg protein	Sulfurisphaera tokodaii
copper	0.56 nmol/mg protein	Sulfurisphaera tokodaii
copper	0.76 nmol/mg protein	Sulfurisphaera tokodaii
Iron	10.5 nmol/mg protein	Sulfurisphaera tokodaii
Iron	18.6 nmol/mg protein	Sulfurisphaera tokodaii
Iron	26.2 nmol/mg protein	Sulfurisphaera tokodaii
Molybdenum	2.9 nmol/mg protein	Sulfurisphaera tokodaii
Molybdenum	4.46 nmol/mg protein	Sulfurisphaera tokodaii
Molybdenum	5.5 nmol/mg protein	Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	-	Sulfurisphaera tokodaii
21000	-	-	Sulfurisphaera tokodaii
29000	-	-	Sulfurisphaera tokodaii
30000	-	-	Sulfurisphaera tokodaii
81000	-	-	Sulfurisphaera tokodaii
82000	-	-	Sulfurisphaera tokodaii
83000	-	-	Sulfurisphaera tokodaii
85000	-	gel filtration	Sulfurisphaera tokodaii
200000	-	gel filtration	Sulfurisphaera tokodaii
372000	-	gel filtration	Sulfurisphaera tokodaii

Organism

Organism	UniProt	Comment	Textmining
Sulfurisphaera tokodaii	F9VNL4 and F9VNL6 and F9VNL5	F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7	-
Sulfurisphaera tokodaii	Q96XN5 and Q974U9 and Q974V0	Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561	-
Sulfurisphaera tokodaii	Q96Y29 and Q974U9 and Q974V0	Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561	-
Sulfurisphaera tokodaii DSM 16993	F9VNL4 and F9VNL6 and F9VNL5	F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7	-
Sulfurisphaera tokodaii DSM 16993	Q96XN5 and Q974U9 and Q974V0	Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561	-
Sulfurisphaera tokodaii DSM 16993	Q96Y29 and Q974U9 and Q974V0	Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561	-

Renatured (Commentary)

Renatured (Comment)	Organism
purified enzyme is dark red in color	Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetaldehyde + 2,6-dichlorophenolindophenol + H2O	-	Sulfurisphaera tokodaii	acetate + reduced 2,6-dichlorophenolindophenol	-	?
acetaldehyde + 2,6-dichlorophenolindophenol + H2O	-	Sulfurisphaera tokodaii DSM 16993	acetate + reduced 2,6-dichlorophenolindophenol	-	?
D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol	-	Sulfurisphaera tokodaii	D-glycerate + reduced 2,6-dichlorophenolindophenol	-	?
D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol	-	Sulfurisphaera tokodaii DSM 16993	D-glycerate + reduced 2,6-dichlorophenolindophenol	-	?
D-glyceraldehyde + H2O + methyl viologen	-	Sulfurisphaera tokodaii	D-glycerate + reduced methyl viologen	-	?
D-glyceraldehyde + H2O + methyl viologen	-	Sulfurisphaera tokodaii DSM 16993	D-glycerate + reduced methyl viologen	-	?
D-glyceraldehyde-3-phosphate + H2O + methyl viologen	-	Sulfurisphaera tokodaii	3-phospho-D-glycerate + reduced methyl viologen	-	?
D-glyceraldehyde-3-phosphate + H2O + methyl viologen	-	Sulfurisphaera tokodaii DSM 16993	3-phospho-D-glycerate + reduced methyl viologen	-	?
indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O	-	Sulfurisphaera tokodaii	indoleacetate + reduced 2,6-dichlorophenolindophenol	-	?
indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O	-	Sulfurisphaera tokodaii DSM 16993	indoleacetate + reduced 2,6-dichlorophenolindophenol	-	?
indolepyruvate + 2,6-dichlorophenolindophenol + H2O	-	Sulfurisphaera tokodaii	? + reduced 2,6-dichlorophenolindophenol	-	?

Subunits

Subunits	Comment	Organism
dodecamer	4 * 83000, gene product ST1781, plus 4 * 29000, gene product ST1783, plus 4 * 20000, gene product ST1782. Isoform Gaor1 is a tetramer of the gene products ST1781/ST1783/ST1782 hetero-trimer	Sulfurisphaera tokodaii
heterohexamer	2 * 81000, gene product ST2339, plus 2 * 30000, gene product ST0562, plus 2 * 21000, gene product ST0561. Isoform Gaor1 is a dimer of the gene products ST1781/ST1783/ST1782 heterotrimer	Sulfurisphaera tokodaii
heterotrimer	1 * 82000, gene product ST2484, plus 1 * 30000, gene product ST0562, plus 1 * 21000, gene product ST0561	Sulfurisphaera tokodaii

Synonyms

Synonyms	Comment	Organism
Gaor1	-	Sulfurisphaera tokodaii
Gaor2	-	Sulfurisphaera tokodaii
Gaor3	-	Sulfurisphaera tokodaii
ST0561	-	Sulfurisphaera tokodaii
ST0562	-	Sulfurisphaera tokodaii
ST1781	-	Sulfurisphaera tokodaii
ST1782	-	Sulfurisphaera tokodaii
ST1783	-	Sulfurisphaera tokodaii
ST2339	-	Sulfurisphaera tokodaii
ST2484	-	Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.43	-	D-Glyceraldehyde-3-phosphate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.65	-	D-Glyceraldehyde-3-phosphate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.72	-	D-glyceraldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
0.98	-	D-Glyceraldehyde-3-phosphate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
2.77	-	Indoleacetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
3.23	-	acetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
3.38	-	acetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
3.46	-	Indoleacetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
3.58	-	D-glyceraldehyde	cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C	Sulfurisphaera tokodaii
3.58	-	Indoleacetaldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
3.78	-	D-glyceraldehyde	cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C	Sulfurisphaera tokodaii
5.23	-	D-glyceraldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
11	-	Indolepyruvate	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
11.2	-	D-glyceraldehyde	cosubstrate methyl viologen, pH 7.0, 70°C	Sulfurisphaera tokodaii
50.5	-	D-glyceraldehyde	cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C	Sulfurisphaera tokodaii

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Sulfurisphaera tokodaii
molybdopterin cytosine dinucleotide	-	Sulfurisphaera tokodaii
[2Fe-2S]-center	small subunit contains two [2Fe-2S] clusters	Sulfurisphaera tokodaii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Sulfurisphaera tokodaii	isoelectric focusing	-	6.2
Sulfurisphaera tokodaii	isoelectric focusing	-	6.3
Sulfurisphaera tokodaii	isoelectric focusing	-	6.9

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Release 2023.1 (January 2023)