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Literature summary for 1.2.98.1 extracted from

  • Kato, N.; Shirakawa, K.; Kobayashi, H.; Sakazawa, C.
    The dismutation of aldehydes by a bacterial enzyme (1983), Agric. Biol. Chem., 47, 39-46.
No PubMed abstract available
Search for more literature for 1.2.98.1

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
 Pseudomonas putida
FeCl3
-
 Pseudomonas putida
HgCl2
-
 Pseudomonas putida
iodoacetate
-
 Pseudomonas putida
p-chloromercuribenzoate
-
 Pseudomonas putida
ZnSO4
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 formaldehyde
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Pseudomonas putida
220000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 F61
-
Pseudomonas putida F61
-
 F61
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
193
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-propanal + H2O + propanal
-
 Pseudomonas putida pyruvate + propanol
-
 ?
acetaldehyde + H2O
-
 Pseudomonas putida acetate + ethanol
-
 ?
formaldehyde + H2O dismutation Pseudomonas putida formate + methanol
-
 ?
formaldehyde + H2O dismutation Pseudomonas putida F61 formate + methanol
-
 ?
formaldehyde + H2O + acrolein cross-dismutation Pseudomonas putida formate + allyl alcohol
-
 ?
formaldehyde + H2O + acrolein cross-dismutation Pseudomonas putida F61 formate + allyl alcohol
-
 ?
formaldehyde + H2O + butyraldehyde cross-dismutation Pseudomonas putida formate + butanol
-
 ?
formaldehyde + H2O + butyraldehyde cross-dismutation Pseudomonas putida F61 formate + butanol
-
 ?
formaldehyde + H2O + crotonaldehyde cross-dismutation Pseudomonas putida formate + crotonyl alcohol
-
 ?
formaldehyde + H2O + ethanal
-
 Pseudomonas putida formate + ethanol
-
 ?
formaldehyde + H2O + isobutyraldehyde cross-dismutation Pseudomonas putida formate + isobutanol
-
 ?
formaldehyde + H2O + isobutyraldehyde cross-dismutation Pseudomonas putida F61 formate + isobutanol
-
 ?
formaldehyde + H2O + propionaldehyde cross-dismutation Pseudomonas putida formate + propanol
-
 ?
formaldehyde + H2O + propionaldehyde cross-dismutation Pseudomonas putida F61 formate + propanol
-
 ?
pyruvaldehyde + H2O
-
 Pseudomonas putida pyruvate + methylglycol
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 10 min, no loss of activity Pseudomonas putida
30
-
 10 min, 21% loss of activity Pseudomonas putida
35
-
 10 min, 50% loss of activity Pseudomonas putida
45
-
 10 min, 82% loss of activity Pseudomonas putida
50
-
 10 min, 100% loss of activity Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 pH 5: about 10% of activity maximum, pH 9.5: about 50% of activity maximum, no activity at pH 10 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7
-
 Pseudomonas putida