BRENDA - Enzyme Database show
show all sequences of 1.2.98.1

Formaldehyde dismutase, a novel NAD-binding oxidoreductase from Pseudomonas putida F61

Kato, N.; Yamagami, T.; Shimao, M.; Sakazawa, C.; Eur. J. Biochem. 156, 59-64 (1986)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
Pseudomonas putida F61
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
HgCl2
-
Pseudomonas putida
additional information
not inhibited by AMP, ADP, ATP and N-methylnicotinamide chloride
Pseudomonas putida
pyrazole
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
1-butanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
0.0032
-
1-propanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
0.02
-
ethanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
0.04
-
methanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zinc
each subunit of the tetramer contains 2 mol zinc per mol
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
4 * 44000, SDS-PAGE
Pseudomonas putida
180000
-
gel filtration
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
F61
-
Pseudomonas putida F61
-
F61
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + p-nitroso-N,N-dimethylaniline
96.2% of activity with ethanol or methanol
390488
Pseudomonas putida
n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
1-butanol + p-nitroso-N,N-dimethylaniline
96.2% of activity with ethanol or methanol
390488
Pseudomonas putida F61
n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
1-pentanol + p-nitroso-N,N-dimethylaniline
9.62% of activity with ethanol
390488
Pseudomonas putida
n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
1-pentanol + p-nitroso-N,N-dimethylaniline
9.62% of activity with ethanol
390488
Pseudomonas putida F61
n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
1-propanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
1-propanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
ethanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
ethanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
ethanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
ethanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
formaldehyde + H2O
dismutation
390488
Pseudomonas putida
formate + methanol
-
390488
Pseudomonas putida
?
formaldehyde + H2O
dismutation
390488
Pseudomonas putida F61
formate + methanol
-
390488
Pseudomonas putida F61
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 44000, SDS-PAGE
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
each subunit contains 1 mol NADH per mol, binds firmly at each active site, enzyme-bound NAD(H) is reduced and oxidized only by the added second substrate (alcohol, aldehyde) not by exogenous electron acceptor
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
each subunit contains 1 mol NADH per mol, binds firmly at each active site, enzyme-bound NAD(H) is reduced and oxidized only by the added second substrate (alcohol, aldehyde) not by exogenous electron acceptor
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
Pseudomonas putida F61
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
HgCl2
-
Pseudomonas putida
additional information
not inhibited by AMP, ADP, ATP and N-methylnicotinamide chloride
Pseudomonas putida
pyrazole
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
1-butanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
0.0032
-
1-propanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
0.02
-
ethanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
0.04
-
methanol
+ p-nitroso-N,N-dimethylaniline
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zinc
each subunit of the tetramer contains 2 mol zinc per mol
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
4 * 44000, SDS-PAGE
Pseudomonas putida
180000
-
gel filtration
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + p-nitroso-N,N-dimethylaniline
96.2% of activity with ethanol or methanol
390488
Pseudomonas putida
n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
1-butanol + p-nitroso-N,N-dimethylaniline
96.2% of activity with ethanol or methanol
390488
Pseudomonas putida F61
n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
1-pentanol + p-nitroso-N,N-dimethylaniline
9.62% of activity with ethanol
390488
Pseudomonas putida
n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
1-pentanol + p-nitroso-N,N-dimethylaniline
9.62% of activity with ethanol
390488
Pseudomonas putida F61
n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
1-propanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
1-propanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
ethanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
ethanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida
?
ethanol + p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
ethanal + reduced p-nitroso-N,N-dimethylaniline
-
390488
Pseudomonas putida F61
?
formaldehyde + H2O
dismutation
390488
Pseudomonas putida
formate + methanol
-
390488
Pseudomonas putida
?
formaldehyde + H2O
dismutation
390488
Pseudomonas putida F61
formate + methanol
-
390488
Pseudomonas putida F61
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 44000, SDS-PAGE
Pseudomonas putida
Other publictions for EC 1.2.98.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740788
Blaschke
Development of a simplified pu ...
Pseudomonas putida, Pseudomonas putida J3
J. Biotechnol.
241
69-75
2017
-
-
1
-
-
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
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-
2
1
-
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-
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-
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-
-
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1
-
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1
2
-
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1
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740186
Yonemitsu
Biodegradation of high concent ...
Methylobacterium sp., Methylobacterium sp. FD1
Biosci. Biotechnol. Biochem.
80
2264-2270
2016
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
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1
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-
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-
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-
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-
2
-
-
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-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
709849
Park
Identification and functional ...
Mycobacterium sp., Mycobacterium sp. DSM 3803
Microbiology
156
463-471
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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3
-
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1
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-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
712174
Alka
Substrate profiling and aldehy ...
no activity in Rattus norvegicus
Int. J. Biochem. Cell Biol.
42
2012-2018
2010
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
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-
-
-
-
-
-
-
655056
Riis
Formate-stimulated oxidation o ...
Pseudomonas putida, Pseudomonas putida 9816
Biosci. Biotechnol. Biochem.
67
684-690
2003
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
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4
-
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2
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4
-
-
-
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-
-
-
-
-
-
-
-
-
-
390495
Yanase
Effects of groESL coexpression ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
66
85-91
2002
-
-
1
1
-
-
-
-
1
1
2
-
-
3
-
-
1
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
1
2
-
-
-
-
1
-
-
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390494
Svensson
Aldehyde dismutase activity of ...
Homo sapiens
FEBS Lett.
394
217-220
1996
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
2
-
-
-
-
-
-
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3
-
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-
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-
1
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3
-
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1
-
2
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
390493
Yanase
Cloning, sequence analysis, an ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
59
197-202
1995
-
-
1
-
-
-
-
-
-
1
2
-
-
5
-
-
1
-
-
-
1
-
1
1
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-
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-
1
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-
1
1
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-
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1
2
-
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-
-
1
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-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390492
Adroer
Mechanism of formaldehyde biod ...
Pseudomonas putida, Pseudomonas putida A2
Appl. Microbiol. Biotechnol.
33
217-220
1990
-
-
-
-
-
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-
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2
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2
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1
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4
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2
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1
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4
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-
-
-
-
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-
390490
Mason
In vivo enzymology: a deuteriu ...
Pseudomonas putida, Pseudomonas putida F61, Staphylococcus aureus
Biochemistry
28
2160-2168
1989
-
-
-
-
-
-
-
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3
-
11
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13
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3
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13
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-
390489
Kato
-
Formate production from methan ...
Pseudomonas putida, Pseudomonas putida F61
Appl. Microbiol. Biotechnol.
27
567-571
1988
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1
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1
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7
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2
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1
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1
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2
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390488
Kato
Formaldehyde dismutase, a nove ...
Pseudomonas putida, Pseudomonas putida F61
Eur. J. Biochem.
156
59-64
1986
-
-
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1
-
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3
4
-
1
2
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8
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1
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10
1
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1
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1
1
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3
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4
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1
2
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1
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10
1
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-
-
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-
390487
Kato
-
Properties of formaldehyde dis ...
Pseudomonas putida, Pseudomonas putida F61
Agric. Biol. Chem.
48
2017-2023
1984
-
-
-
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5
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2
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7
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1
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1
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22
1
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2
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5
2
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2
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1
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1
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22
1
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390491
Kato
-
The dismutation of aldehydes b ...
Pseudomonas putida, Pseudomonas putida F61
Agric. Biol. Chem.
47
39-46
1983
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6
1
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2
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7
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1
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15
1
1
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5
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1
1
1
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6
-
1
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2
-
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1
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15
1
1
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5
-
1
1
1
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