KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
CoA | pH 9.0, 80°C | Thermococcus profundus | |
0.018 | - |
phenylpyruvate | in 50 mM TAPS buffer (pH 9.0), at 80°C | Thermococcus profundus | |
0.06 | - |
oxidized ferredoxin | in 50 mM TAPS buffer (pH 9.0), at 80°C | Thermococcus profundus | |
0.108 | - |
phenylpyruvate | pH 9.0, 80°C | Thermococcus profundus | |
0.13 | - |
4-hydroxyphenylpyruvate | pH 9.0, 80°C | Thermococcus profundus | |
0.13 | - |
4-hydroxyphenylpyruvate | in 50 mM TAPS buffer (pH 9.0), at 80°C | Thermococcus profundus | |
0.22 | - |
Indolepyruvate | pH 9.0, 80°C | Thermococcus profundus | |
0.22 | - |
(indol-3-yl)pyruvate | in 50 mM TAPS buffer (pH 9.0), at 80°C | Thermococcus profundus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
2 * 67000 + 2 * 21000, SDS-PAGE | Thermococcus profundus |
21000 | - |
2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE | Thermococcus profundus |
67000 | - |
2 * 67000 + 2 * 21000, SDS-PAGE | Thermococcus profundus |
67000 | - |
2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE | Thermococcus profundus |
155000 | - |
sedimentation equilibrium and sedimentation velocity experiments | Thermococcus profundus |
170000 | - |
gel filtration | Thermococcus profundus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Thermococcus profundus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Thermococcus profundus DSM DT5432 | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus profundus | - |
- |
- |
Thermococcus profundus DSM DT5432 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
25°C, aerobic conditions, half-life 7 h | Thermococcus profundus |
the enzyme is oxygen-sensitive with a half-life of 7 h at 25°C under aerobic conditions | Thermococcus profundus |
Purification (Comment) | Organism |
---|---|
purification under extremely anaerobic conditions in presence of 2mM dithiothreitol and 2mM sodium dithionite to protect against oxygen attack, and also 1mM MgSO4,1mM thiamine diphosphate and 0.2M NaCl, additives which have been found to stabilize | Thermococcus profundus |
under extremely anaerobic conditions with all buffers containing 2 mM dithiothreitol and 2 mM dithiothreitol. Q Sepharose column chromatography, hydroxyapatite column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration | Thermococcus profundus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.01 | - |
cell-free extract, in 50 mM TAPS buffer (pH 9.0), at 80°C | Thermococcus profundus |
121 | - |
pH 9.0, 80°C | Thermococcus profundus |
121 | - |
after 120fold purification, in 50 mM TAPS buffer (pH 9.0), at 80°C | Thermococcus profundus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus DSM DT5432 | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus | ? + CO2 + reduced ferredoxin + H+ | - |
? | |
4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus DSM DT5432 | ? + CO2 + reduced ferredoxin + H+ | - |
? | |
4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
indolepyruvate + CoA + oxidized methylviologen | - |
Thermococcus profundus | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
indolepyruvate + CoA + oxidized methylviologen | - |
Thermococcus profundus DSM DT5432 | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus profundus DSM DT5432 | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
phenylpyruvate + CoA + oxidized methyl viologen | - |
Thermococcus profundus | phenylacetyl-CoA + CO2 + reduced methyl viologen + H+ | - |
? | |
phenylpyruvate + CoA + oxidized methyl viologen | - |
Thermococcus profundus DSM DT5432 | phenylacetyl-CoA + CO2 + reduced methyl viologen + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 67000 + 2 * 21000, SDS-PAGE | Thermococcus profundus |
tetramer | 2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE | Thermococcus profundus |
Synonyms | Comment | Organism |
---|---|---|
IOR | - |
Thermococcus profundus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
- |
Thermococcus profundus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | negligible activity | Thermococcus profundus |
60 | 100 | dramatic increase of activity | Thermococcus profundus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 110 | a slight decrease in activity is observed below 90°C for at least 4 h. The enzyme exhibits a half-life of 62 min at 100°C, 40 min at 103°C, 20 min at 105°C, 5 min at 108°C, and less than 5 min at 110°C | Thermococcus profundus |
100 | - |
half-life 62 min | Thermococcus profundus |
105 | - |
half-life 20 min | Thermococcus profundus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | Km-value 0.06 mM | Thermococcus profundus | |
[4Fe-4S]-center | - |
Thermococcus profundus |