Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.7.8 extracted from

  • Ozawa, Y.; Siddiqui, M.A.; Takahashi, Y.; Urushiyama, A.; Ohmori, D.; Yamakura, F.; Arisaka, F.; Imai, T.
    Indolepyruvate ferredoxin oxidoreductase: An oxygen-sensitive iron-sulfur enzyme from the hyperthermophilic archaeon Thermococcus profundus (2012), J. Biosci. Bioeng., 114, 23-27.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
CoA pH 9.0, 80°C Thermococcus profundus
0.018
-
phenylpyruvate in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
0.06
-
oxidized ferredoxin in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
0.108
-
phenylpyruvate pH 9.0, 80°C Thermococcus profundus
0.13
-
4-hydroxyphenylpyruvate pH 9.0, 80°C Thermococcus profundus
0.13
-
4-hydroxyphenylpyruvate in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
0.22
-
Indolepyruvate pH 9.0, 80°C Thermococcus profundus
0.22
-
(indol-3-yl)pyruvate in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
2 * 67000 + 2 * 21000, SDS-PAGE Thermococcus profundus
21000
-
2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE Thermococcus profundus
67000
-
2 * 67000 + 2 * 21000, SDS-PAGE Thermococcus profundus
67000
-
2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE Thermococcus profundus
155000
-
sedimentation equilibrium and sedimentation velocity experiments Thermococcus profundus
170000
-
gel filtration Thermococcus profundus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin Thermococcus profundus
-
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
?
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin Thermococcus profundus DSM DT5432
-
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus profundus
-
-
-
Thermococcus profundus DSM DT5432
-
-
-

Oxidation Stability

Oxidation Stability Organism
25°C, aerobic conditions, half-life 7 h Thermococcus profundus
the enzyme is oxygen-sensitive with a half-life of 7 h at 25°C under aerobic conditions Thermococcus profundus

Purification (Commentary)

Purification (Comment) Organism
purification under extremely anaerobic conditions in presence of 2mM dithiothreitol and 2mM sodium dithionite to protect against oxygen attack, and also 1mM MgSO4,1mM thiamine diphosphate and 0.2M NaCl, additives which have been found to stabilize Thermococcus profundus
under extremely anaerobic conditions with all buffers containing 2 mM dithiothreitol and 2 mM dithiothreitol. Q Sepharose column chromatography, hydroxyapatite column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration Thermococcus profundus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.01
-
cell-free extract, in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus
121
-
pH 9.0, 80°C Thermococcus profundus
121
-
after 120fold purification, in 50 mM TAPS buffer (pH 9.0), at 80°C Thermococcus profundus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
?
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus DSM DT5432 S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin + H+
-
?
4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus ? + CO2 + reduced ferredoxin + H+
-
?
4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus DSM DT5432 ? + CO2 + reduced ferredoxin + H+
-
?
4-hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin + H+
-
?
indolepyruvate + CoA + oxidized methylviologen
-
Thermococcus profundus indoleacetyl-CoA + CO2 + reduced methylviologen
-
?
indolepyruvate + CoA + oxidized methylviologen
-
Thermococcus profundus DSM DT5432 indoleacetyl-CoA + CO2 + reduced methylviologen
-
?
phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus profundus DSM DT5432 phenylacetyl-CoA + CO2 + reduced ferredoxin
-
?
phenylpyruvate + CoA + oxidized methyl viologen
-
Thermococcus profundus phenylacetyl-CoA + CO2 + reduced methyl viologen + H+
-
?
phenylpyruvate + CoA + oxidized methyl viologen
-
Thermococcus profundus DSM DT5432 phenylacetyl-CoA + CO2 + reduced methyl viologen + H+
-
?

Subunits

Subunits Comment Organism
heterotetramer 2 * 67000 + 2 * 21000, SDS-PAGE Thermococcus profundus
tetramer 2 * 67000, alpha-subunit, plus 2 * 21000, beta-subunit, SDS-PAGE Thermococcus profundus

Synonyms

Synonyms Comment Organism
IOR
-
Thermococcus profundus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
105
-
-
Thermococcus profundus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30 negligible activity Thermococcus profundus
60 100 dramatic increase of activity Thermococcus profundus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 110 a slight decrease in activity is observed below 90°C for at least 4 h. The enzyme exhibits a half-life of 62 min at 100°C, 40 min at 103°C, 20 min at 105°C, 5 min at 108°C, and less than 5 min at 110°C Thermococcus profundus
100
-
half-life 62 min Thermococcus profundus
105
-
half-life 20 min Thermococcus profundus

Cofactor

Cofactor Comment Organism Structure
Ferredoxin Km-value 0.06 mM Thermococcus profundus
[4Fe-4S]-center
-
Thermococcus profundus